Natural distribution of 5 bacteria associated with periodontal disease.
Natural distribution of 5 bacteria associated with periodontal disease.
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与牙周病相关的 5 种细菌的自然分布。
DOI：
10.1111/j.1600-051x.1993.tb00694.x
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发表时间：
1993
影响因子：
6.7
通讯作者：
Fischer,G
中科院分区：
文献类型：
--
作者：
Wolff,LF;Aeppli,DM;Pihlstrom,B;Anderson,L;Stoltenberg,J;Osborn,J;Hardie,N;Shelburne,C;Fischer,G
本研究的目的是了解龈下菌斑中5种细菌病原菌的患病率、分布、相互关系及探测深度。从938名受试者的6905个部位收集斑块。采用细菌浓度荧光免疫分析法和细菌特异性单克隆抗体测定p的存在和水平。gingivalis (Pg)。actinomycetemcomitans (Aa), P。媒介物(π),E。carrodens andF (Ec)。每个空斑样本中的核核体（Fn）。受试者中Pg的患病率最低（32%），Ec的患病率最高（49%）。这些细菌种类基于位点的频率分布范围从Pg的10.3%到Ec的18.7%。Pi和Ec是在同一受试者中最常见的细菌组合（27.2%）。64.0%的站点未检测到5种细菌中的任何一种，20.2%的站点仅检测到5种细菌中的一种。其余15.8%的站点至少存在2种细菌。细菌种类的检测水平与探测深度基本呈线性相关。比值比为3.9 （Pg）。3.0 (Aa), 4.0 （Pi）2.7 （Ec）和2.8 （Fn）发现高水平的这些细菌病原体在bbb50 mm探测深度（p≤0.01）。有特定细菌的受试者在没有特定细菌的磨牙部位的平均探测深度比没有特定细菌的受试者大（p≤0.01）。观察到这5种细菌经常栖息在牙龈下环境中，但与晚期疾病无关，这表明在疾病可能发生进展之前，除了“致病菌”外，还需要一个易感宿主。
The purpose of this study was to determine the prevalence and distribution of 5 bacterial pathogens in subgingival plaque, their relationship with each other and probing depth. Plaque was collected from 6905 sites in 938 subjects. A bacterial concentration fluorescence immunoassay and bacterial specific monoclonal antibodies were used to determine the presence and level ofP. gingivalis(Pg),A. actinomycetemcomitans(Aa),P. intermedia(Pi),E. carrodens(Ec) andF. nucleatum(Fn) in each plaque sample. The prevalence in subjects was lowest for Pg (32%) and highest for Ec (49%). The site‐based frequency distribution of these bacterial species ranged from 10.3% for Pg to 18.7% for Ec. Pi and Ec were the bacterial combination most often found together in a subject (27.2%). While 64.0% of the sites were without any of the 5 bacterial species evaluated, 20.2% had only 1 of the 5 bacterial species evaluated. The remaining 15.8% of sites had at least 2 bacteria species present. There was a general linear association of the detection level of bacterial species and probing depth. The odds ratios were 3.9 (Pg). 3.0 (Aa), 4.0 (Pi). 2.7 (Ec) and 2.8 (Fn) of finding high levels of these bacterial pathogens at > 5 mm probing depth (p≤ 0.01). Mean probing depth at molar sites without a specific bacteria was greater (p≤ 0.01) insubjects wisha specific bacterium compared to molar sites insubjects withoutthe bacteria. The observation that these 5 bacterial species frequently inhabit the subgingival environment, yet are not associated with advanced disease, suggest that a susceptible host is required, in addition to a “pathogenic bacteria”, before disease progression may occur.