Altered Growth, Pigmentation, and Antimicrobial Susceptibility Properties of Staphylococcus aureus Due to Loss of the Major Cold Shock Gene cspB
Altered Growth, Pigmentation, and Antimicrobial Susceptibility Properties of Staphylococcus aureus Due to Loss of the Major Cold Shock Gene cspB
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DOI：
10.1128/aac.01786-09
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发表时间：
2010-06-01
影响因子：
4.9
通讯作者：
Shafer, William M.
中科院分区：
文献类型：
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作者：
Duval, Brea D.;Mathew, Anselmo;Shafer, William M.
在金黄色葡萄球菌菌株COL（一种耐甲氧西林的临床分离物）的主要冷休克基因cspB中进行插入突变，产生了一种突变，表现出对冷休克的反应能力降低和金黄色葡萄球菌小菌落变异的许多表型特征：37℃下生长缺陷，色素沉着减少，以及对许多抗菌素的易感性水平改变。特别是，cspB零突变体对氨基糖苷类、甲氧苄啶-磺胺甲恶唑和百草枯的抗性增加，对达托霉素、替柯planin和甲氧西林的敏感性增加。除了对甲氧西林的敏感性增加，这是由于I型葡萄球菌盒染色体mec元件的完全丢失，当cspB以反式表达时，这些特性通过互补恢复到野生型水平。综上所述，我们的研究结果将金黄色葡萄球菌的应激反应蛋白（CspB）与重要的表型特性联系起来，包括对某些抗菌素的耐药性。
An insertional mutation made in the major cold shock gene cspB in Staphylococcus aureus strain COL, a methicillin-resistant clinical isolate, yielded a mutant that displayed a reduced capacity to respond to cold shock and many phenotypic characteristics of S. aureus small-colony variants: a growth defect at 37 degrees C, a reduction in pigmentation, and altered levels of susceptibility to many antimicrobials. In particular, a cspB null mutant displayed increased resistance to aminoglycosides, trimethoprim-sulfamethoxazole, and paraquat and increased susceptibility to daptomycin, teicoplanin, and methicillin. With the exception of the increased susceptibility to methicillin, which was due to a complete loss of the type I staphylococcal cassette chromosome mec element, these properties were restored to wild-type levels by complementation when cspB was expressed in trans. Taken together, our results link a stress response protein (CspB) of S. aureus to important phenotypic properties that include resistance to certain antimicrobials.