In vivo leukocyte cytokine mRNA responses to biomaterials are dependent on surface chemistry
In vivo leukocyte cytokine mRNA responses to biomaterials are dependent on surface chemistry
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DOI：
10.1002/jbm.a.10425
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发表时间：
2003-02-01
影响因子：
4.9
通讯作者：
Anderson, JM
中科院分区：
文献类型：
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作者：
Brodbeck, WG;Voskerician, G;Anderson, JM
使用体内小鼠笼植入系统来确定白细胞细胞因子mRNA对植入生物材料的反应是否依赖于表面化学。将显示各种化学性质（疏水性、亲水性、阴离子和阳离子）的表面置于不锈钢笼中并皮下植入。半定量RT-PCR分析显示，亲水性表面显示粘附细胞的促炎细胞因子IL-6和IL-8以及促伤口愈合细胞因子IL-10和TGF-β的表达降低，并且促炎细胞因子IL-1 β和促伤口愈合细胞因子IL-13的mRNA水平在周围渗出细胞中降低。粘附细胞和渗出液细胞对疏水性、阴离子和阳离子表面的细胞因子反应相似，表明在最早时间点出现强烈炎症反应，随后在较晚时间点出现伤口愈合反应。然而，在每个相应的时间点，未观察到任何表面或空融合器的渗出液细胞类型或水平存在差异，表明其各自的生物相容性。这些研究确定亲水性表面化学物质通过降低粘附于生物材料以及存在于周围渗出物中的炎性细胞的炎性和伤口愈合细胞因子的表达，对体内白细胞细胞细胞因子应答具有显著影响。(C)2002 Wiley Periodicals，Inc.
An in vivo mouse cage implant system was used to determine whether leukocyte cytokine mRNA responses to implanted biomaterials were dependent on surface chemistry. Surfaces displaying various chemistries (hydrophobic, hydrophilic, anionic, and cationic) were placed into stainless steel cages and implanted subcutaneously. Semiquantitative RT-PCR analyses revealed that hydrophilic surfaces showed a decreased expression of proinflammatory cytokines, IL-6 and IL-8, and pro-wound healing cytokines, IL-10 and TGF-beta by adherent cells, and mRNA levels of the proinflammatory cytokine, IL-1beta, and the pro-wound healing cytokine IL-13 were decreased in surrounding, exudate cells. Cytokine responses by adherent and exudate cells to hydrophobic, anionic and cationic surfaces were similar and indicative of a strong inflammatory response at the earliest time point followed by a wound healing response at later time points. However, no differences in the types or levels of exudate cells for any of the surfaces or the empty cage at each of the respective time points were observed, indicating their respective biocompatibility. These studies identify hydrophilic surface chemistries as having significant effects on leukocyte cytokine responses in vivo by decreasing the expression of inflammatory and wound healing cytokines by inflammatory cells adherent to the biomaterial as well as present in the surrounding exudate. (C) 2002 Wiley Periodicals, Inc.