Development of an epitope conservancy analysis tool to facilitate the design of epitope-based diagnostics and vaccines.
Development of an epitope conservancy analysis tool to facilitate the design of epitope-based diagnostics and vaccines.
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DOI：
10.1186/1471-2105-8-361
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发表时间：
2007-09-26
影响因子：
3
通讯作者：
Sette, Alessandro
中科院分区：
文献类型：
--
作者：
Bui, Huynh-Hoa;Sidney, John;Li, Wei;Fusseder, Nicolas;Sette, Alessandro
在基于表位的疫苗环境中，保守表位的使用将预期提供比源自高度可变的基因组区域的表位更广泛的跨多个毒株或甚至物种的保护。相反，在诊断和疾病监测环境中，例如，对给定病原体菌株具有特异性的表位可用于监测对该特定感染性菌株的应答。在这两种情况下，与所考虑的表位的保护程度有关的具体信息是至关重要的。为了帮助选择具有所需保守程度的表位，我们开发了一种新的工具来确定给定蛋白质序列组内表位的变异性。该工具是作为免疫表位数据库和分析资源（IEDB）的一个组件实现的，可以直接访问。开发了表位保守性分析工具，用于分析表位的变异性或保守性。该工具是用户友好的，预计将有助于基于表位的疫苗和诊断的设计。
In an epitope-based vaccine setting, the use of conserved epitopes would be expected to provide broader protection across multiple strains, or even species, than epitopes derived from highly variable genome regions. Conversely, in a diagnostic and disease monitoring setting, epitopes that are specific to a given pathogen strain, for example, can be used to monitor responses to that particular infectious strain. In both cases, concrete information pertaining to the degree of conservancy of the epitope(s) considered is crucial. To assist in the selection of epitopes with the desired degree of conservation, we have developed a new tool to determine the variability of epitopes within a given set of protein sequences. The tool was implemented as a component of the Immune Epitope Database and Analysis Resources (IEDB), and is directly accessible at . An epitope conservancy analysis tool was developed to analyze the variability or conservation of epitopes. The tool is user friendly, and is expected to aid in the design of epitope-based vaccines and diagnostics.