An Improved Mesocotyl Elongation Assay for the Rapid Identification and Characterization of Strigolactone-Related Rice Mutants
An Improved Mesocotyl Elongation Assay for the Rapid Identification and Characterization of Strigolactone-Related Rice Mutants
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DOI：
10.3390/agronomy9040208
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发表时间：
2019-04
期刊：
影响因子：
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通讯作者：
S. Patil;S. A. Zafar;M. Uzair;Jinfeng Zhao;Jingjing Fang;Xueyong Li
中科院分区：
文献类型：
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作者：
S. Patil;S. A. Zafar;M. Uzair;Jinfeng Zhao;Jingjing Fang;Xueyong Li
独脚金内酯（SL）是一类重要的植物激素，参与植物生长和寄主-寄生物相互作用的多种发育活动。尽管对这一途径的理解已经取得了实质性进展，但其作用机制仍然难以捉摸，特别是与其他植物激素和下游靶标的相互作用。本文利用独脚金内酯在水稻（Oryza sativa L.）中胚轴伸长作为鉴定和表征新的发育突变体的形态学标记。我们观察到，深播种子发育较长的中胚轴相比，在黑暗条件下的表面生长的种子。在此基础上，我们发展了一种以玻璃容器和蛭石为生长介质的中胚轴伸长方法。与黑暗条件下表面生长的种子相比，改良深播系统中的中胚轴伸长导致许多倍的增加。SLs类似物rac-GR 24的外部应用挽救了SLs合成缺陷突变体中的伸长的中胚轴表型，但不是信号转导突变体，证明了其在生理实验中的适用性。改进的中胚轴伸长测定法可用作表征和鉴定SL或其相关途径中的抑制子/增强子和新的发育突变体的快速方法，节省了大量的时间和空间。
Strigolactones (SLs) constitute an important class of plant hormones involved in diverse developmental activities in plant growth and host-parasite interaction. Although substantial progress has been made to understand this pathway, the mechanism of action is still elusive especially with its interaction with other phytohormones and downstream targets. Here we have utilized the negative role of strigolactones in rice (Oryza sativa L.) mesocotyl elongation as a morphological marker for the identification and characterization of new developmental mutants. We observed that deep sown seeds develop longer mesocotyl compared with the surface-grown seeds in the dark condition. Based on this observation, we have developed a method to access mesocotyl elongation consisting of the glass vessel and vermiculite as a growth media. Mesocotyl elongation in the modified deep sown system results in a many-fold increase compared to the surface-grown seeds in the dark condition. External application of SLs analog rac-GR24 rescued the elongated mesocotyl phenotype in the mutant defective in SLs synthesis but not the signaling mutant, demonstrating its applicability in the physiological experiments. The modified mesocotyl elongation assay can be used as a rapid method for characterization and identification of suppressors/enhancers and new developmental mutants in the SLs or its associated pathway saving a huge amount of time and space.