Demonstration of a dynamic, transcription-dependent organization of pre-mRNA splicing factors in polytene nuclei
Demonstration of a dynamic, transcription-dependent organization of pre-mRNA splicing factors in polytene nuclei
复制标题
DOI：
10.1083/jcb.133.5.929
复制
发表时间：
1996-06-01
影响因子：
7.8
通讯作者：
Wieslander, L
中科院分区：
文献类型：
--
作者：
Bauren, G;Jiang, WQ;Wieslander, L
我们描述了动态组织的前mRNA剪接因子在完整的多线核的双翅目摇蚊tentans。snRNP和SR非snRNP剪接因子过量存在，主要分布在整个染色质间。大约10%的U2 snRNP和SR非snRNP剪接因子与染色体相关，在与RNA结合的活性基因位点高度富集。我们证明，剪接因子是专门招募到一个定义的基因在生理条件下诱导转录。同时，剪接因子离开转录关闭的基因位点。我们还证明，在一般转录抑制时，剪接因子从活性基因位点重新分布到染色质间。我们的研究结果表明，动态核内组织的剪接因子和转录之间的紧密联系和核内组织的剪接机器。
We describe the dynamic organization of pre-mRNA splicing factors in the intact polytene nuclei of the dipteran Chironomus tentans. The snRNPs and an SR non-snRNP splicing factor are present in excess, mainly distributed throughout the interchromatin. Approximately 10% of the U2 snRNP and an SR non-snRNP splicing factor are associated with the chromosomes, highly enriched in active gene loci where they are bound to RNA. We demonstrate that the splicing factors are specifically recruited to a defined gene upon induction of transcription during physiological conditions. Concomitantly, the splicing factors leave gene loci in which transcription is turned off, We also demonstrated that upon general transcription inhibition, the splicing factors redistribute from active gene loci to the interchromatin. Our findings demonstrate the dynamic intranuclear organization of splicing factors and a tight linkage between transcription and the intranuclear organization of the splicing machinery.