Evidence for an FSH-releasing factor in the posterior portion of the rat median eminence.
Evidence for an FSH-releasing factor in the posterior portion of the rat median eminence.
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大鼠正中隆起后部存在 FSH 释放因子的证据。
DOI：
10.1016/0024-3205(83)90739-7
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发表时间：
1983
期刊：
影响因子：
6.1
通讯作者：
McCann,SM
中科院分区：
文献类型：
--
作者：
Mizunuma,H;Samson,WK;Lumpkin,MD;McCann,SM
为了检验FSH释放因子可能包含在正中隆起（ME）后部的假设，分别从成年雄性大鼠脑中取出ME的前半部分（aME）和ME的后半部分（pME），并在0.2 N乙酸中提取。LH和FSH释放活性的提取物进行了测定，在体外培养8个半垂体从成年雄性大鼠在5个组织当量的剂量为6小时，并确定放射免疫测定LH和FSH释放到培养基中。在两个实验中，由aME提取物诱导的LH释放显著大于由pME诱导的LH释放，而aME和pME提取物之间的FSH释放没有差异。当测试1和2 ng合成LHRH时，在该系统中注意到FSH释放的显著剂量相关增加，这表明该测定法对不同量的LHRH的FSH释放作用敏感。提取物中免疫反应性LHRH的含量几乎是aME中pME的两倍。因此，结果表明，pME具有比其LHRH含量更大的FSH释放活性。额外的FSH释放活性可能是由于与LHRH不同的FSH释放因子。
To test the hypothesis that an FSH-releasing factor might be contained within the posterior portion of the median eminence (ME), the anterior half of the ME (aME) and the posterior half of the ME (pME) were removed separately from the brains of adult male rats and extracted in 0.2 N acetic acid. LH and FSH-releasing activities of the extracts were measured in vitro by incubating 8 hemipituitaries from adult male rats for 6 h at a dose of 5 tissue equivalents and determining the radioimmunoassayable LH and FSH released into the medium. LH release induced by the aME extracts was significantly greater than that induced by the pME in both experiments, whereas there were no differences in FSH release between aME and pME extracts. A significant dose-related increase in FSH release was noted in this system when 1 and 2 ng of synthetic LHRH were tested which indicates that the assay was sensitive to different amounts of LHRH with regard to FSH-releasing action. The content of immunoreactive LHRH in the extracts was almost twice as high in the aME as in the pME. Therefore, the results indicate that the pME has greater FSH-releasing activity than can be accounted for by its content of LHRH. The additional FSH-releasing activity is presumably due to an FSH-releasing factor distinct from LHRH.