Elevated Ratios of Type I/III Collagen in the Lungs of Chronically Ventilated Neonates with Respiratory Distress
Elevated Ratios of Type I/III Collagen in the Lungs of Chronically Ventilated Neonates with Respiratory Distress
复制标题
患有呼吸窘迫的长期通气新生儿肺部 I/III 型胶原比例升高
作者：
C. Shoemaker;K. Reiser;B. Goetzman;J. Last
摘要。在实验性肺纤维化动物的肺中观察到I型胶原的合成增加，导致肺中I型与III型胶原的比率增加。在死于特发性肺纤维化和成人呼吸窘迫综合征的人的肺中观察到胶原类型比率的类似变化。在这项研究中，肺胶原类型的比率进行了检查，在婴儿急性和慢性肺部疾病。新生儿右下叶的组织是在死后获得的。通过用溴化氰溶解肺胶原蛋白并通过柱层析和聚丙烯酰胺凝胶电泳分离所得肽混合物来定量特定的胶原蛋白类型。对于这些测定，使用两对独立的标记肽计算每个肺样品的I/III型胶原的比率。在某些情况下，还定量了这些相同肺样品中Y型胶原与III型胶原的比率。我们观察到，在死亡前诊断为慢性肺病的婴儿中，I/III型胶原蛋白的比例显著增加，通常在呼吸窘迫综合征之前。我们还观察到两个婴儿的胶原蛋白类型的比例有很大的变化，他们可能有继发于宫内肺部疾病的肺纤维化。这些数据表明，可能有几个子集的婴儿呼吸窘迫综合征，每一个有不同的预后。
ABSTRACT. Increased synthesis of type I collagen, leading to increased ratios of type I to type III collagen in the lungs, has been observed in the lungs of animals with experimental pulmonary fibrosis. Similar changes in collagen type ratios have been observed in lungs of humans dying of idiopathic pulmonary fibrosis and of adult respiratory distress syndrome. In this study, lung collagen type ratios were examined in infants with acute and chronic lung disease. Tissue from the right lower lobes of neonates was obtained post mortem. Specific collagen types were quantitated by solubilization of lung collagen with CNBr and fractionation of the resulting mixture of peptides by column chromatography and polyacrylamide gel electrophoresis. Ratios of type I/III collagen were calculated for each lung sample using two independent pairs of marker peptides for these determinations. In some cases the ratio of type Y to type III collagen in these same lung samples was also quantitated. We observed a significant increase in the ratio of type I/III collagen in infants with a premortem diagnosis of chronic lung disease, usually preceded by respiratory distress syndrome. We also observed two infants with large changes in collagen type ratios who might have had pulmonary fibroplasia secondary to intrauterine lung disease. These data suggest that there may be several subsets of infants with respiratory distress syndrome, each having a different prognosis.