Reduction in adipocyte ATP by lipolytic agents: relation to intracellular free fatty acid accumulation.
Reduction in adipocyte ATP by lipolytic agents: relation to intracellular free fatty acid accumulation.
复制标题
脂肪分解剂减少脂肪细胞 ATP：与细胞内游离脂肪酸积累的关系。
DOI：
10.1016/s0022-2275(20)39531-6
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发表时间：
1971
影响因子：
6.5
通讯作者：
Mitchell L. Halperin
中科院分区：
文献类型：
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作者：
Aubie Angel;K. Desai;Mitchell L. Halperin
肾上腺素、去甲肾上腺素、ACTH和双丁酰3′，5 ′-环AMP在60分钟孵育期间降低脂肪细胞ATP水平;葡萄糖显示保护作用。脂肪细胞ATP水平的降低不能仅仅归因于：直接激素效应、代谢底物缺乏、腺苷酸环化酶的激活与ATP消耗、腺嘌呤核苷酸从细胞中的损失或孵育期间细胞的损失、脂解速率本身或FFA或甘油的细胞外积累。为了确定细胞内FFA积累是否是致病因素，在脂肪酸刺激的脂解过程中测量细胞内FFA水平。这是通过使用蔗糖-U-14 C作为细胞外空间的标记物来校正细胞外白蛋白结合FFA对细胞的污染来实现的。这些实验表明，脂肪细胞ATP的下降与培养基白蛋白的FFA饱和度和脂肪细胞内FFA的进行性积累相关。此外，上述葡萄糖的保护作用与细胞内游离脂肪酸的显着减少相比，细胞外游离脂肪酸pool.On这些研究的基础上，结合那些在文献中，它的结论是，在体外脂肪分解剂对脂肪细胞ATP水平的影响是ATP合成受损（解偶联氧化磷酸化）在面对正常或增加的ATP消耗的净结果。
Epinephrine, norepinephrine, ACTH, and dibutyryl 3′,5′-cyclic AMP reduced adipocyte ATP levels during 60 min incubation; glucose displayed a protective effect. The reduction in adipocyte ATP levels could not be attributed solely to: a direct hormone effect, deficiency in metabolic substrate, activation of adenyl cyclase with ATP consumption, loss of adenine nucleotide from the cell or loss of cells during incubation, lipolytic rate per se, or extracellular accumulation of FFA or glycerol.To determine whether intracellular FFA accumulation was a causative factor, intracellular FFA levels were measured during hormone-stimulated lipolysis. This was accomplished by using sucrose-U-14C as a marker for the extracellular space to correct for contamination of cells by extracellular albumin-bound FFA. These experiments showed that the fall in adipocyte ATP correlated with FFA saturation of medium albumin and progressive accumulation of FFA within the adipocyte. Furthermore, the protective effect of glucose noted above was associated with a marked reduction in intracellular FFA as compared to the extracellular FFA pool.On the basis of these studies, combined with those in the literature, it is concluded that in vitro effects of lipolytic agents on adipocyte ATP levels are the net result of impaired ATP synthesis (uncoupled oxidative phosphorylation) in the face of normal or augmented ATP consumption.
DOI：
--
发表时间：
1966-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Rodbell
通讯作者：
M. Rodbell