Quantifying cerebral blood flow in an adult pig ischemia model by a depth-resolved dynamic contrast-enhanced optical method
Quantifying cerebral blood flow in an adult pig ischemia model by a depth-resolved dynamic contrast-enhanced optical method
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DOI：
10.1016/j.neuroimage.2014.03.023
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发表时间：
2014-07-01
期刊：
影响因子：
5.7
通讯作者：
Lawrence, Keith St.
中科院分区：
文献类型：
--
作者：
Elliott, Jonathan T.;Diop, Mamadou;Lawrence, Keith St.
动态对比度增强（DCE）近红外（NIR）方法已被提出用于床旁监测脑血流（CBF）。这些方法主要集中在定性方法，因为头皮污染阻碍定量。在这项研究中，我们证明，准确的CBF测量可以通过分析多距离时间分辨DCE数据与组合动力学去卷积光学重建（KDOR）方法。在正常碳酸血症、低碳酸血症和缺血期间，对成年猪（n = 8）进行多距离时间分辨DCE-NIR测量。使用KDOR方法从DCE-NIR测量值计算CBF。为了验证，在每种条件下通过CT独立测量CBF。两种技术之间的平均CBF差异为-1.7 mL/100 g/min，95%置信区间为16.3 mL/100 g/min和12.9 mL/100 g/min;组回归分析显示两种技术之间的高度一致（斜率= 1.06 +/- 0.08，y轴截距= -4.37 +/- 4.33 mL/100 g/min，p < 0.001）。误差分析的结果表明，需要很少的先验信息来恢复CBF，由于分析方法的鲁棒性和时间分辨近红外光谱直接表征脑外组织的光学特性的能力（其中模型失配是有害的）。这项研究的结果表明，DCE-NIR方法是一种微创和便携式的手段，确定绝对血流动力学在神经重症监护患者。(C)2014 Elsevier Inc. All rights reserved.
Dynamic contrast-enhanced (DCE) near-infrared (NIR) methods have been proposed for bedside monitoring of cerebral blood flow (CBF). These methods have primarily focused on qualitative approaches since scalp contamination hinders quantification. In this study, we demonstrate that accurate CBF measurements can be obtained by analyzing multi-distance time-resolved DCE data with a combined kinetic deconvolution optical reconstruction (KDOR) method. Multi-distance time-resolved DCE-NIR measurements were made in adult pigs (n = 8) during normocapnia, hypocapnia and ischemia. The KDOR method was used to calculate CBF from the DCE-NIR measurements. For validation, CBF was measured independently by CT under each condition. The mean CBF difference between the techniques was - 1.7 mL/100 g/min with 95% confidence intervals of 16.3 and 12.9 mL/100 g/min; group regression analysis revealed a strong agreement between the two techniques (slope = 1.06 +/- 0.08, y-intercept = -4.37 +/- 4.33 mL/100 g/min, p < 0.001). The results of an error analysis suggest that little a priori information is needed to recover CBF, due to the robustness of the analytical method and the ability of time-resolved NIR to directly characterize the optical properties of the extracerebral tissue (where model mismatch is deleterious). The findings of this study suggest that the DCE-NIR approach presented is a minimally invasive and portable means of determining absolute hemodynamics in neurocritical care patients. (C) 2014 Elsevier Inc. All rights reserved.