Polyphosphate-accumulating organisms in full-scale tropical wastewater treatment plants use diverse carbon sources
Polyphosphate-accumulating organisms in full-scale tropical wastewater treatment plants use diverse carbon sources
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大型热带废水处理厂中的聚磷酸盐积累生物使用多种碳源
DOI：
10.1016/j.watres.2018.11.011
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发表时间：
2019
期刊：
影响因子：
12.8
通讯作者：
Wuertz Stefan
中科院分区：
文献类型：
--
作者：
Qiu Guanglei;Zuniga-Montanez Rogelio;Law Yingyu;Thi Sara Swa;Thi Quynh Ngoc Nguyen;Eganathan Kaliyamoorthy;Liu Xianghui;Nielsen Per H.;Williams Rohan B. H.;Wuertz Stefan
Enhanced biological phosphorus removal (EBPR) is considered challenging in the tropics, based on a great number of laboratory-based studies showing that the polyphosphate-accumulating organism(PAO)CandidatusAccumulibacter does not compete well with glycogen accumulating organisms (GAOs) at temperatures above 25 °C. Yet limited information is available on the PAO community and the metabolic capabilities in full-scale EBPR systems operating at high temperature. We studied the composition of the key functional PAO communities in three full-scale wastewater treatment plants (WWTPs) with high in-situ EBPR activity in Singapore, their EBPR-associated carbon usage characteristics, and the relationship between carbon usage and community composition. Each plant had a signature community composed of diverse putative PAOs with multiple operational taxonomic units (OTUs) affiliated toCa.Accumulibacter,Tetrasphaeraspp.,DechloromonasandCa.Obscuribacter. Despite the differences in community composition, ex-situ anaerobic phosphorus (P)-release tests with 24 organic compounds from five categories (including four sugars, three alcohols, three volatile fatty acids (VFAs), eight amino acids and six other carboxylic acids) showed that a wide range of organic compounds could potentially contribute to EBPR. VFAs induced the highest P release (12.0–18.2 mg P/g MLSS for acetate with a P release-to-carbon uptake (P:C) ratio of 0.35–0.66 mol P/mol C, 9.4–18.5 mg P/g MLSS for propionate with a P:C ratio of 0.38–0.60, and 9.5–17.3 mg P/g MLSS for n-butyrate), followed by some carboxylic acids (10.1–18.1 mg P/g MLSS for pyruvate, 4.5–11.7 mg P/g MLSS for lactate and 3.7–12.4 mg P/g MLSS for fumarate) and amino acids (3.66–7.33 mg P/g MLSS for glutamate with a P:C ratio of 0.16–0.43 mol P/mol C, and 4.01–7.37 mg P/g MLSS for aspartate with a P:C ratio of 0.17–0.48 mol P/mol C). P-release profiles (induced by different carbon sources) correlated closely with PAO community composition. High micro-diversity was observed within theCa.Accumulibacter lineage, which represented the most abundant PAOs. The total population ofCa.Accumulibacter taxa was highly correlated with P-release induced by VFAs, highlighting the latter's importance in tropical EBPR systems. There was a strong link between the relative abundance of individualCa.Accumulibacter OTUs and the extent of P release induced by distinct carbon sources (e.g., OTU 81 and amino acids, and OTU 246 and ethanol), suggesting niche differentiation amongCa.Accumulibacter taxa. A diverse PAO community and the ability to use numerous organic compounds are considered key factors for stable EBPR in full-scale plants at elevated temperatures.
