Inhibition of Mycobacterial Growth In Vitro following Primary but Not Secondary Vaccination with Mycobacterium bovis BCG
Inhibition of Mycobacterial Growth In Vitro following Primary but Not Secondary Vaccination with Mycobacterium bovis BCG
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DOI：
10.1128/cvi.00427-13
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发表时间：
2013-11-01
影响因子：
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通讯作者：
McShane, Helen
中科院分区：
文献类型：
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作者：
Fletcher, Helen A.;Tanner, Rachel;McShane, Helen
尽管牛分枝杆菌卡介苗疫苗得到广泛使用，但每年仍有超过 900 万新发结核病病例，迫切需要更好的结核病疫苗。结核病候选疫苗的选择部分基于抗原特异性γ干扰素（IFN-γ）反应的检测来进行评估。对从接受试验性结核疫苗免疫的受试者获得的血液样本中分枝杆菌生长的测量可能与体内疫苗功效具有更好的体外相关性。我们对 30 名英国成年人进行了一项临床研究，随访了 6 个月，以评估基于全血和新型外周血单核细胞 (PBMC) 的分枝杆菌生长抑制测定的能力，以测量对初次接种和再次接种 BCG 的反应。使用冷冻保存的 PBMC，我们观察到初次接种疫苗后分枝杆菌生长抑制显着改善，但再次接种 BCG 后生长抑制没有改善（P
Despite the widespread use of the Mycobacterium bovis BCG vaccine, there are more than 9 million new cases of tuberculosis (TB) every year, and there is an urgent need for better TB vaccines. TB vaccine candidates are selected for evaluation based in part on the detection of an antigen-specific gamma interferon (IFN-gamma) response. The measurement of mycobacterial growth in blood specimens obtained from subjects immunized with investigational TB vaccines may be a better in vitro correlate of in vivo vaccine efficacy. We performed a clinical study with 30 United Kingdom adults who were followed for 6 months to evaluate the abilities of both a whole-blood- and a novel peripheral blood mononuclear cell (PBMC)-based mycobacterial growth inhibition assay to measure a response to primary vaccination and revaccination with BCG. Using cryopreserved PBMCs, we observed a significant improvement in mycobacterial growth inhibition following primary vaccination but no improvement in growth inhibition following revaccination with BCG (P