Smilax china L. rhizome extract inhibits nuclear factor-κB and induces apoptosis in ovarian cancer cells
Smilax china L. rhizome extract inhibits nuclear factor-κB and induces apoptosis in ovarian cancer cells
复制标题
DOI：
10.1007/s11655-014-1788-9
复制
发表时间：
2015-12-01
影响因子：
2.9
通讯作者：
Hua Xiao-li
中科院分区：
文献类型：
--
作者：
Hu Li-ling;Chen Dong-sheng;Hua Xiao-li
目的：研究菝葜提取物的抗肿瘤作用及其相关机制。用不同浓度的黄芪提取物（SCRE）处理卵巢癌细胞A2780，并与对照组进行比较。采用CCK-8法检测细胞生长; EdU掺入法检测细胞增殖;碘化丙啶染色法检测细胞周期; Annexin V-异硫氰酸荧光素/碘化丙啶法检测细胞凋亡;核因子-κ B的细胞分布（NF-κ B）; NF-κ B、caspase-3、聚腺苷二磷酸（ADP）-核糖聚合酶（PARP）、Bcl-2相关X蛋白（Bax）、细胞凋亡抑制因子（cIAP）-1的蛋白水平，Western blotting检测X连锁凋亡抑制蛋白（XIAP）、B细胞淋巴瘤-特大（Bcl-XL）、B细胞淋巴瘤-2（Bcl-2）和AKT; CCK-8法检测SCRE与顺铂或阿霉素联合应用对A2780细胞增殖的影响，SCRE对A2780细胞增殖有抑制作用，且呈剂量依赖性（P <0.05，P < 0.01），通过激活caspase-3、PARP和Bax，使细胞阻滞于G（2）/M期，诱导细胞凋亡。SCRE治疗还与NF-κ B的抑制和Bcl-2、Bcl-XL、cIAP-1、XIAP和AKT的下调相关。SCRE能提高A2780细胞对顺铂和阿霉素的化疗敏感性（P < 0.01），能有效抑制NF-κ B B，诱导细胞凋亡，降低卵巢癌细胞对顺铂和阿霉素的耐药性，这可能是其治疗卵巢癌的分子基础。
To study the antitumor effects and associated mechanisms of extract of the Smilax china L. rhizome (SCR) on ovarian cancer cells.Ovarian cancer cells A2780 were treated with different concentrations of SCR extract (SCRE), and compared with controls. Effects on cell growth were evaluated by cell counting kit-8 (CCK-8) assay; proliferation effects by EdU incorporation assay; cell cycle by propidium iodide staining; apoptosis by annexin V-fluorescein isothiocyanate/propidium iodide; cellular distribution of nuclear factor-kappa B (NF-kappa B) by immunofluorescence; protein levels of NF-kappa B, caspase-3, poly-adenosine diphosphate (ADP)-ribose polymerase (PARP), Bcl-2-associated X protein (Bax), cellular inhibitor of apoptosis (cIAP)-1, anti-X-linked inhibitor of apoptosis protein (XIAP), B-cell lymphoma-extra large (Bcl-XL), B-cell lymphoma-2 (Bcl-2) and AKT by Western blotting; and effects of SCRE combined with cisplatin or adriamycin on A2780 cells by CCK-8 assay.SCRE suppressed A2780 cell proliferation in a dose-dependent manner (P < 0.05,P < 0.01), arrested cells in G(2)/M phase and induced apoptosis by activating caspase-3, PARP and Bax. SCRE treatment also correlated with inhibition of NF-kappa B and downregulation of Bcl-2, Bcl-XL, cIAP-1, XIAP and AKT. SCRE can promote chemosensitivity to cisplatin and adriamycin in A2780 cells (P < 0.01).SCR effectively inhibits NF-kappa B, induces apoptosis and reduces chemoresistance to cisplatin and adriamycin in ovarian cancer cells, which might be its molecular basis for treating ovarian cancer.