Peroxidase-like activity of water-soluble cupric oxide nanoparticles and its analytical application for detection of hydrogen peroxide and glucose
Peroxidase-like activity of water-soluble cupric oxide nanoparticles and its analytical application for detection of hydrogen peroxide and glucose
复制标题
水溶性氧化铜纳米颗粒的类过氧化物酶活性及其在过氧化氢和葡萄糖检测中的分析应用
DOI：
10.1039/c2an35072f
复制
发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Xia, Xing-Hua
中科院分区：
文献类型：
--
作者：
Chen, Wei;Chen, Juan;Xia, Xing-Hua
水溶性氧化铜纳米粒子通过快速沉淀法制备，并用作过氧化物酶模拟物，用于过氧化氢和葡萄糖的超灵敏检测。水溶性CuO纳米粒子由于对过氧化氢有较高的亲和力，其催化活性远高于市售CuO纳米粒子。此外，所制备的CuO纳米颗粒在宽范围的pH和温度下是稳定的。这种水胶体悬浮液形式的优异稳定性使得水溶性CuO纳米颗粒更容易在水性体系中应用。基于过氧化氢催化4-氨基安替比林偶联苯酚氧化反应，建立了过氧化氢和葡萄糖的比色分析法。该分析平台不仅证实了水溶性氧化铜纳米粒子的内在过氧化物酶样活性，而且显示了其在环境化学、生物技术和医学领域的巨大应用潜力。
Water-soluble cupric oxide nanoparticles are fabricated via a quick-precipitation method and used as peroxidase mimetics for ultrasensitive detection of hydrogen peroxide and glucose. The water-soluble CuO nanoparticles show much higher catalytic activity than that of commercial CuO nanoparticles due to their higher affinity to hydrogen peroxide. In addition, the as-prepared CuO nanoparticles are stable over a wide range of pH and temperature. This excellent stability in the form of aqueous colloidal suspensions makes the application of the water-soluble CuO nanoparticles easier in aqueous systems. A colorimetric assay for hydrogen peroxide and glucose has been established based on the catalytic oxidation of phenol coupled with 4-amino-atipyrine by the action of hydrogen peroxide. This analytical platform not only confirms the intrinsic peroxidase-like activity of the water-soluble cupric oxide nanoparticles, but also shows its great potential applications in environmental chemistry, biotechnology and medicine.