The measurement of protein synthesis in biological systems.
The measurement of protein synthesis in biological systems.
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生物系统中蛋白质合成的测量。
DOI：
10.1016/0024-3205(82)90300-9
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发表时间：
1982
期刊：
影响因子：
6.1
通讯作者：
Watkins,CA
中科院分区：
文献类型：
--
作者：
Rannels,DE;Wartell,SA;Watkins,CA
使用放射性前体对肺和其他组织的新陈代谢进行量化的尝试可能会因对前体新陈代谢、分区和特定放射性的不适当假设而产生重大错误。本文回顾了可能使这种测量复杂化的误差的类型和大小，提供了使用放射性氨基酸估计蛋白质合成速率的实验的具体数据。简要讨论了这些观察结果对其他代谢系统的适用性，以便更全面地认识到使用放射性同位素对实验测量的不完全验证可能导致的误差。
Attempts to quantitate metabolism in the lung and other tissues using radioactive precursors may be subject to significant errors arising from inappropriate assumptions regarding precursor metabolism, compartmentation and specific radioactivity. This article reviews the type and magnitude of error which may complicate such measurements by presenting specific data from experiments using radioactive amino acids to estimate the rate of protein synthesis. The applicability of these observations to other metabolic systems is discussed briefly in order to develop a more general awareness of the errors which may result from incomplete validation of experimental measurements using radioisotopes.