EVIDENCE THAT DISCONTINUOUS DNA-REPLICATION IN ESCHERICHIA-COLI IS PRIMED BY APPROXIMATELY 10 TO 12 RESIDUES OF RNA STARTING WITH A PURINE
EVIDENCE THAT DISCONTINUOUS DNA-REPLICATION IN ESCHERICHIA-COLI IS PRIMED BY APPROXIMATELY 10 TO 12 RESIDUES OF RNA STARTING WITH A PURINE
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DOI：
10.1016/0022-2836(85)90042-7
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发表时间：
1985-01-01
影响因子：
5.6
通讯作者：
OKAZAKI, T
中科院分区：
文献类型：
--
作者：
KITANI, T;YODA, KY;OKAZAKI, T
通过用牛痘鸟苷酸转移酶和[α-32P]GTP在体外特异性标记其5''-末端三(或二)磷酸基团来检测用于大肠杆菌不连续DNA复制的完整引物RNA。 RNase H 或 RNase H 和 DNA 聚合酶 I 两者都有缺陷的突变体积累了.apprx。完整引物 RNA 分别比野生型细胞多 10 倍或 30 倍。引物以嘌呤开始，A 与 G 的比例为 5，最丰富的 5'' 末端二核苷酸序列是 (p)ppA-Pu。完整引物RNA的链长为.apprx。 10-12个核苷酸残基。大肠杆菌引物 RNA 的结构特性与真核引物 RNA 相似。
Intact primer RNA for discontinuous DNA replication of E. coli was detected by specific labeling in vitro of its 5''-terminal tri- (or di-) phosphate group with vaccinia guanylyltransferase and [.alpha.-32P]GTP. A mutant defective either in RNase H or in both RNase H and DNA polymerase I accumulated .apprx. 10 or 30 times more intact primer RNA, respectively, than wild-type cells. The primers started with purine in an A to G ratio of 5 and the most abundant 5''-terminal dinucleotide sequence was (p)ppA-Pu. The chain length of the intact primer RNA was .apprx. 10-12 nucleotide residues. The structural properties of the E. coli primer RNA resemble those of the eukaryotic primer RNA.