Molecular analysis of an enhancin gene in the Lymantria dispar nuclear polyhedrosis virus
Molecular analysis of an enhancin gene in the Lymantria dispar nuclear polyhedrosis virus
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DOI：
10.1128/jvi.71.11.8133-8140.1997
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发表时间：
1997-11-01
影响因子：
5.4
通讯作者：
Slavicek, JM
中科院分区：
文献类型：
--
作者：
Bischoff, DS;Slavicek, JM
舞毒蛾核型多角体病毒（LdMNPV）基因已被鉴定，其编码能够增强其它杆状病毒感染的颗粒病毒（GV）增强蛋白的同源物。增强蛋白基因已被确定和测序的三种GV，但尚未发现在任何其他核型多角体病毒的日期。LdMNPV增强蛋白基因位于病毒基因组上的67.6和70.1 kbp之间。病毒RNA的北方和引物延伸分析表明，增强蛋白基因转录本在感染后的晚期由共有杆状病毒晚期启动子表达。LdMNPV增强蛋白与粉纹夜蛾（Trichoplusia ni）、单斑拟夜蛾（Pseudaletia unipuncta）和棉铃虫（Helicoverpa armigera）GV的增强蛋白具有29%的氨基酸同一性。所有四种蛋白质都含有金属蛋白酶特有的保守锌结合结构域。构建了重组病毒（enhnncin：：cat），其中通过插入诱变使LdMNPV enhancin基因失活，以确定enhancin蛋白对病毒效力的影响。生物测定结果表明，破坏LdMNPV中的增强蛋白基因导致病毒效力降低。
A Lymantria dispar nuclear polyhedrosis virus (LdMNPV) gene has been identified that encodes a homolog to the granulovirus (GV) enhancin proteins that are capable of enhancing the infection of other baculoviruses. Enhancin genes have been identified and sequenced for three species of GVs but have not been found in any other nuclear polyhedrosis virus to date. The LdMNPV enhancin gene is located between 67.6 and 70.1 kbp on the viral genome. Northern and primer extension analyses of viral RNAs indicate that the enhancin gene transcripts are expressed at late times postinfection from a consensus baculovirus late promoter. The LdMNPV enhancin exhibits 29% amino acid identity to the enhancin proteins of the Trichoplusia ni, Pseudaletia unipuncta, and Helicoverpa armigera GVs. All four proteins contain a conserved zinc-binding domain characteristic of metalloproteases. A recombinant virus (enhnncin::cat) was constructed in which the LdMNPV enhancin gene was inactivated by insertion mutagenesis in order to ascertain the effect of the enhancin protein on viral potency. The bioassay results indicate that disruption of the enhnncin gene in the LdMNPV results in a reduction in viral potency.