Exogenous surfactant changes the phenotype of alveolar macrophages in mice
Exogenous surfactant changes the phenotype of alveolar macrophages in mice
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DOI：
10.1152/ajplung.2001.280.4.l689
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发表时间：
2001-04-01
影响因子：
4.9
通讯作者：
Ikegami, M
中科院分区：
文献类型：
--
作者：
Kramer, BW;Jobe, AH;Ikegami, M
肺泡巨噬细胞是维持表面活性剂稳态所必需的。我们询问表面活性剂处理是否会改变肺泡巨噬细胞的数量，以及当外源性表面活性剂在体内刺激时，肺泡巨噬细胞的表型是否会被激活或凋亡。经120 mg/kg （110 mmol/kg）饱和磷脂酰胆碱重复处理后，小鼠表面活性剂池大小增加。肺泡灌洗恢复的肺泡巨噬细胞数量在第一次给药后减少49%，在第二次和第三次给药后略有增加。高达28.5%的巨噬细胞在12小时内变大并呈泡沫状，其外观正常化。表面活性剂处理未增加凋亡或坏死细胞的百分比。经表面活性剂处理后，肺泡巨噬细胞的CD14、CD16、CD54、CD95及A类I型和II型清道夫受体的表达均未发生变化。在健康小鼠中，表面活性剂处理可使肺泡巨噬细胞的表型瞬间改变为大而泡沫，而表面标记物的活化特征没有变化。
Alveolar macrophages are essential for the maintenance of surfactant homeostasis. We asked whether surfactant treatment would change alveolar macrophage number and whether the alveolar macrophage phenotype would become activated or apoptotic when challenged in vivo with exogenous surfactant. Surfactant pool size in mice was increased by repetitive surfactant treatments containing 120 mg/kg (110 mmol/kg) saturated phosphatidylcholine. The number of alveolar macrophages recovered by alveolar lavage decreased after the first dose by 49% and slightly increased after the second and third doses. Up to 28.5% of the macrophages became large and foamy, and their appearance normalized within 12 h. Surfactant treatment did not increase the percent of apoptotic or necrotic cells. The alveolar macrophages were not activated as indicated by no change in expression of CD14, CD16, CD54, CD95, and scavenger receptor class A types I and II after surfactant treatment. Surfactant treatment in healthy mice transiently changed the phenotype of alveolar macrophages to large and foamy without indications of changes in the surface markers characteristic of activation.