Transmembrane potential changes during phagocytosis in rat alveolar macrophages
Transmembrane potential changes during phagocytosis in rat alveolar macrophages
复制标题
大鼠肺泡巨噬细胞吞噬过程中跨膜电位的变化
DOI：
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发表时间：
1981
影响因子：
5.6
通讯作者：
V. Castranova
中科院分区：
文献类型：
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作者：
P. R. Miles;L. Bowman;V. Castranova
进行研究以测量在细胞暴露于酵母聚糖颗粒或膜扰动剂佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）期间大鼠肺泡巨噬细胞跨膜电位的变化，并确定膜电位的变化是否与超氧阴离子释放有关。细胞暴露于酵母聚糖或PMA导致膜去极化，这先于超氧阴离子释放。此外，去极化的幅度取决于酵母聚糖或PMA的浓度。在肺泡巨噬细胞暴露于酵母聚糖水平增加的过程中，释放的超氧化物的量增加，去极化的幅度也增加。在含有150 mMK+的培养基中孵育细胞，该培养基引起膜去极化，导致从静息细胞释放超氧化物，并且从暴露于酵母聚糖的细胞释放的超氧化物的量减少。这些结果表明，超氧阴离子从大鼠肺泡巨噬细胞释放与膜去极化，并建议跨膜电位的变化可能作为一个信号，启动细胞的吞噬反应。
Studies were carried out to measure changes in the transmembrane potential of rat alveolar macrophages during exposure of the cells to zymosan particles or to the membrane perturbant, phorbol‐12‐myristate‐13‐acetate (PMA), and to determine if changes in membrane potential are related to superoxide anion release. Exposure of the cells to either zymosan or PMA leads to membrane depolarization, which precedes superoxide anion release. Furthermore, the magnitude of the depolarization is dependent upon the concentration of either zymosan or PMA. During exposure of the alveolar macrophages to increasing levels of zymosan, there is an increase in the amount of superoxide released as well as an increase in the magnitude of the depolarization. Incubation of the cells in medium containing 150 mMK+, a medium which causes membrane depolarization, leads to superoxide release from resting cells and a decrease in the amount of superoxide released from cells exposed to zymosan. These results indicate that release of superoxide anion from rat alveolar macrophages is related to membrane depolarization and suggest that the transmembrane potential change may act as a signal to initiate the phagocytotic responses of the cells.