Mineral Trioxide Aggregate Inhibits Osteoclastic Bone Resorption
Mineral Trioxide Aggregate Inhibits Osteoclastic Bone Resorption
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DOI：
10.1177/0022034511407335
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发表时间：
2011-07-01
影响因子：
7.6
通讯作者：
Jimi, E.
中科院分区：
文献类型：
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作者：
Hashiguchi, D.;Fukushima, H.;Jimi, E.
三氧化矿物聚合物（MTA）是一种常用的根管修复材料，在基础和临床研究中均很有用，MTA对成骨细胞分化的影响已被明确。然而，MTA对骨细胞骨吸收的影响尚未完全了解。因此，本研究的目的是使用在原代成骨细胞和骨髓细胞的共培养物中形成的破骨细胞来检查MTA溶液在调节破骨细胞骨吸收活性中的作用。MTA溶液剂量依赖性地减少破骨细胞形成的凹陷总面积。20%MTA处理引起的骨吸收减少是由于骨细胞骨吸收活性的抑制，而对破骨细胞数量没有影响。20%MTA溶液通过降低c-Src的磷酸化和激酶活性以及组织蛋白酶K和MMP-9的mRNA表达来破坏肌动蛋白环的形成，肌动蛋白环是骨细胞骨吸收的标志。高浓度的MTA溶液（50%）通过增加促凋亡蛋白的仅BH 3（Bcl-2同源性）家族的成员Bim的表达诱导破骨细胞凋亡。总而言之，我们的结果表明MTA是一种适用于多种临床情况的有用的填充材料，因为它既刺激成骨细胞分化又抑制骨吸收。
Mineral trioxide aggregate (MTA), a commonly used endodontic repair material, is useful for both basic and clinical research, and the effect of MTA on osteoblast differentiation has been well-defined. However, the effects of MTA on osteoclastic bone resorption are not fully understood. Hence, the aim of this study is to examine the effect of MTA solution in the regulation of osteoclast bone-resorbing activity using osteoclasts formed in co-cultures of primary osteoblasts and bone marrow cells. MTA solution dose-dependently reduced the total area of pits formed by osteoclasts. The reduction of resorption induced by 20% MTA treatment was due to inhibition of osteoclastic bone-resorbing activity and had no effect on osteoclast number. A 20% MTA solution disrupted actin ring formation, a marker of osteoclastic bone resorption, by reducing phosphorylation and kinase activity of c-Src, and mRNA expressions of cathepsin K and mmp-9. A high concentration of MTA solution (50%) induced apoptosis of osteoclasts by increasing the expression of Bim, a member of the BH3-only (Bcl-2 homology) family of pro-apoptotic proteins. Taken together, our results suggest that MTA is a useful retrofilling material for several clinical situations because it both stimulates osteoblast differentiation and inhibits bone resorption.