Establishment of a visible peritoneal micrometastatic model from a gastric adenocarcinoma cell line by green fluorescent protein.
Establishment of a visible peritoneal micrometastatic model from a gastric adenocarcinoma cell line by green fluorescent protein.
复制标题
绿色荧光蛋白建立胃腺癌细胞系可见腹膜微转移模型。
DOI：
--
复制
发表时间：
2000
影响因子：
5.2
通讯作者：
M. Monden
中科院分区：
文献类型：
--
作者：
K. Kaneko;M. Yano;T. Tsujinaka;S. Morita;M. Taniguchi;Y. Fujiwara;Y. Doki;M. Inoue;H. Shiozaki;M. Monden
为建立可见的腹膜微转移模型，将增强型绿色荧光蛋白(EGFP)表达载体导入人胃癌细胞系MKN-45。将高表达的EGFP细胞注射到裸鼠的腹膜腔内。术后第1、4、7天分别取腹膜和腹部脏器，荧光体视显微镜观察未发现的腹膜微转移灶。在这个模型中，可以检测到小到单个细胞的微转移。该腹膜微转移模型有望成为研究胃癌转移的有用工具。
To establish a visible peritoneal micrometastatic model, an enhanced green fluorescent protein (EGFP) expressing plasmid vector was transfected into the gastric cancer cell line, MKN-45. Highly expressing EGFP cells were injected into the peritoneal cavity of nude mice. Then, the peritoneum and abdominal organs were harvested and observed on days 1, 4 and 7. Fluorescence stereomicroscopy identified peritoneal micrometastases that had been undetected by stereomicroscopy. Micrometastases as small as a single cell are detectable in this model. This peritoneal micrometastatic model should be a useful tool for research on metastasis of gastric cancer.
细菌 lacZ 基因作为检测肿瘤进展过程中微转移形成的高度敏感标记。
影响因子：
11.2
作者：
Lin,WC;Pretlow,TP;Pretlow2nd,TG;Culp,LA
通讯作者：
Culp,LA