Effects of Echinococcus multilocularis miR-71 mimics on murine macrophage RAW264.7 cells
Effects of Echinococcus multilocularis miR-71 mimics on murine macrophage RAW264.7 cells
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多房棘球绦虫miR-71模拟物对小鼠巨噬细胞RAW264.7细胞的影响
DOI：
10.1016/j.intimp.2016.03.015
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发表时间：
2016-05-01
影响因子：
5.6
通讯作者：
Cao, Jianping
中科院分区：
文献类型：
--
作者：
Zheng, Yadong;Guo, Xiaola;Cao, Jianping
microRNA（miRNAs）是一类小的调节性非编码RNA，其有助于在感染期间激活宿主-病原体串扰。在蠕虫中，miR-71是高度保守的，最近在线虫外泌体以及感染的人和小鼠的血清和/或体液中检测到。然而，miR-71在感染过程中的作用仍然很难表征。在此，我们发现，Ago 1和Ago 4，编码小RNA诱导的沉默复合物（RISC）的关键成分，在小鼠巨噬细胞RAW264.7细胞转染的多房棘球绦虫miR-71（emu-miR-71）模拟上调。使用miRNA PCR阵列，在转染的细胞中，参与炎症或自身免疫的84种miRNA中没有一种显著上调或下调（p > 0.05）。虽然它不影响处理的细胞产生IL-10在LPS和IFN-γ处理后12 h，模拟物显著抑制NO的产生（p > 0.05）（P < 0.01），确定了另一种潜在的机制，寄生虫可以仔细调节宿主的NO水平。这些发现表明，寄生虫来源的miR-71进入宿主后可影响巨噬细胞的功能，这可能为寄生虫与宿主之间相互作用的研究提供了一个令人兴奋的方向。(C)2016爱思唯尔B. V.保留所有权利。
The microRNAs (miRNAs) are a class of small regulatory non-coding RNA that contributes to the activation of host-pathogen cross-talk during infection. In helminthes, miR-71 is highly conserved and it has recently been detected in nematode exosomes, as well as in the sera and/or fluids of infected humans and mice. However, the role of miR-71 during infection remains poorly characterized. Herein, we show that Ago1 and Ago4, which encode key components of the small RNA-induced silencing complex (RISC), were up-regulated in murine macrophage RAW264.7 cells transfected by Echinococcus multilocularis miR-71 (emu-miR-71) mimics. Using a miRNA PCR array, none of the 84 miRNAs involved in inflammation or autoimmunity were significantly up- or down regulated in the transfected cells (p > 0.05). Although it did not influence IL-10 production by the treated cells (p > 0.05), the mimics significantly repressed the production of NO 12 h after treatment with LPS and IFN-gamma (p < 0.01), identifying another potential mechanism whereby parasites can carefully regulate host levels of NO. These findings indicate that the release of parasite-derived miR-71 into hosts can affect the functions of macrophages, and possibly represents an exciting direction for studies of the interplay between parasites and hosts. (C) 2016 Elsevier B.V. All rights reserved.