Isolation of dental pulp stem cells from a single donor and characterization of their ability to differentiate after 2 years of cryopreservation.
Isolation of dental pulp stem cells from a single donor and characterization of their ability to differentiate after 2 years of cryopreservation.
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DOI：
10.15537/smj.2016.5.13615
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发表时间：
2016-05
影响因子：
1.6
通讯作者：
Ashri NY
中科院分区：
文献类型：
--
作者：
Alsulaimani RS;Ajlan SA;Aldahmash AM;Alnabaheen MS;Ashri NY
目的：研究单个供者牙髓干细胞（DPSCs）冻存2年后的活力和分化能力。这项前瞻性研究于2010年10月至2014年2月在沙特阿拉伯利雅得沙特国王大学医学院干细胞研究中心进行。从11名参与者中提取的17颗牙齿被单独处理，以评估产生体外培养细胞所需的最小组织重量。在第4代之前，使用殖民地形成单位测定、免疫荧光染色和双谱系分化来评估细胞的干细胞性。牙髓干细胞冻存2年。培养解冻后的DPSC直至衰老并向成骨、成牙、成脂和成软骨谱系分化。从11名参与者中的6名成功分离出活细胞。这6个培养的细胞系中的3个被鉴定为DPSC。从单个供体成功分离活细胞需要至少0.2 g牙髓组织。解冻后的DPSC成功分化为成骨、成牙、成软骨和成脂肪谱系。解冻后的DPSC在体外可存活至衰老前70天。细胞间无显著差异。在本研究的限制范围内，使用最少2颗拔除的牙齿从同一供体成功分离出牙髓组织中的活细胞。从牙髓组织中分离的细胞并非都具有DPSCs的特征。解冻后的DPSC保持其多谱系分化能力。
To investigate the viability and differentiation capacity of dental pulp stem cells (DPSCs) isolated from single donors after two years of cryopreservation. This prospective study was conducted between October 2010 and February 2014 in the Stem Unit, College of Medicine, King Saud University, Riyadh, Saudi Arabia. Seventeen teeth extracted from 11 participants were processed separately to assess the minimum tissue weight needed to yield cells for culturing in vitro. Cell stemness was evaluated before passage 4 using the colony forming unit assay, immunofluorescence staining, and bi-lineage differentiation. Dental pulp stem cells were cryopreserved for 2 years. Post-thaw DPSCs were cultured until senescence and differentiated toward osteogenic, odontogenic, adipogenic, and chondrogenic lineages. Viable cells were isolated successfully from 6 of the 11 participants. Three of these 6 cultured cell lines were identified as DPSCs. A minimum of 0.2 g of dental pulp tissue was required for successful isolation of viable cells from a single donor. Post-thaw DPSCs successfully differentiated towards osteogenic, odontogenic, chondrogenic, and adipogenic lineages. The post-thaw DPSCs were viable in vitro up to 70 days before senescence. There was no significant difference between the cells. Within the limitations of this investigation, viable cells from dental pulp tissue were isolated successfully from the same donor using a minimum of 2 extracted teeth. Not all isolated cells from harvested dental pulp tissue had the characteristics of DPSCs. Post-thaw DPSCs maintained their multi-lineage differentiation capacity.