CFI 25 Subunit of Cleavage Factor I is Important for Maintaining the Diversity of 3' UTR Lengths in Arabidopsis thaliana (L.) Heynh.
CFI 25 Subunit of Cleavage Factor I is Important for Maintaining the Diversity of 3' UTR Lengths in Arabidopsis thaliana (L.) Heynh.
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切割因子 I 的 CFI 25 亚基对于维持拟南芥 (L.) Heynh 中 3 UTR 长度的多样性非常重要。
DOI：
10.1093/pcp/pcac002
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发表时间：
2022
影响因子：
4.9
通讯作者：
Tsuge Tomohiko
中科院分区：
文献类型：
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作者：
Zhang Xiaojuan;Nomoto Mika;Garcia-Leon Marta;Takahashi Naoki;Kato Mariko;Yura Kei;Umeda Masaaki;Rubio Vicente;Tada Yasuomi;Furumoto Tsuyoshi;Aoyama Takashi;Tsuge Tomohiko
前体mRNA 3 ′端的切割和多聚腺苷酸化通过调节其可翻译性、稳定性和转运至细胞质而对mRNA功能至关重要。切割因子I（CFI）是真核生物中前体mRNA 3 ′末端加工机制的多亚基组分。在此，我们报道了植物CFI 25亚基在维持mRNA的3个末端的多样性方面起着重要作用。拟南芥（Arabidopsis thaliana（L.）嘿含有4个基因编码3个假定的CFI亚基（AtCFI 25、AtCFI 59和AtCFI 68），与哺乳动物CFI亚基直向同源。存在两种与人CFI 25共享同源性的CFI 25旁系同源物（AtCFI 25 a和AtCFI 25 b）。AtCFI 25a的两个无效等位基因表现为莲座叶较小、柱头乳突较长、花药较小、开花较早和育性较低。AtCFI 25b的两个等位基因以及异位表达AtCFI 25a全长cDNA的植株没有表现出明显的形态缺陷。AtCFI 25 a与AtCFI 25 b、AtCFI 68和AtCFI 25 a本身相互作用，表明植物中存在多种形式的CFI。此外，我们还发现AtCFI 25 a功能对于维持编码CFI亚基的转录物的3个末端长度的适当多样性是必不可少的，这表明植物中的CFI机制是自我调节的，AtCFI 25 a在不同程度上对于维持其他基因的3个末端也是重要的。总的来说，AtCFI 25a，而不是AtCFI 25b，似乎在拟南芥发育过程中发挥重要作用，通过维持适当的3个UTR长度的多样性。
Cleavage and polyadenylation at the 3ʹ end of the pre-mRNA is essential for mRNA function, by regulating its translatability, stability and translocation to the cytoplasm. Cleavage factor I (CFI) is a multi-subunit component of the pre-mRNA 3ʹ end processing machinery in eukaryotes. Here, we report that plant CFI 25 subunit of CFI plays an important role in maintaining the diversity of the 3ʹ ends of mRNA. The genome ofArabidopsis thaliana(L.) Heynh. contained four genes encoding three putative CFI subunits (AtCFI 25, AtCFI 59 and AtCFI 68), orthologous to the mammalian CFI subunits. There were two CFI 25 paralogs (AtCFI 25a and AtCFI 25b) that shared homology with human CFI 25. Two null alleles ofAtCFI 25adisplayed smaller rosette leaves, longer stigmatic papilla, smaller anther, earlier flowering and lower fertility compared to wild-type plants. Null alleles ofAtCFI 25b, as well as, plants ectopically expressing full-length cDNA ofAtCFI 25a, displayed no obvious morphological defects. AtCFI 25a was shown to interact with AtCFI 25b, AtCFI 68 and itself, suggesting various forms of CFI in plants. Furthermore, we show thatAtCFI 25afunction was essential for maintaining proper diversity of the 3ʹ end lengths of transcripts coding for CFI subunits, suggesting a self-regulation of the CFI machinery in plants.AtCFI 25awas also important to maintain 3ʹ ends for other genes to different extent. Collectively,AtCFI 25a, but notAtCFI 25b, seemed to play important roles during Arabidopsis development by maintaining proper diversity of the 3ʹ UTR lengths.