ONE OUT OF 5 PERIPHERAL-BLOOD LYMPHOCYTES-B IS ACTIVATED TO HIGH-RATE IG PRODUCTION BY HUMAN ALLOREACTIVE T-CELL CLONES
ONE OUT OF 5 PERIPHERAL-BLOOD LYMPHOCYTES-B IS ACTIVATED TO HIGH-RATE IG PRODUCTION BY HUMAN ALLOREACTIVE T-CELL CLONES
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DOI：
10.1002/eji.1830131008
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发表时间：
1983-01-01
影响因子：
5.4
通讯作者：
LANZAVECCHIA, A
中科院分区：
文献类型：
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作者：
LANZAVECCHIA, A
人同种异体反应性辅助性T细胞克隆是从二次混合淋巴细胞反应中分离出来的，在照射刺激细胞和T细胞生长因子（白细胞介素2）的存在下，通过有限稀释。当与具有相关同种异体抗原的B细胞和巨噬细胞一起培养时，T细胞克隆增殖并诱导强烈的B细胞活化，产生高水平的IG。来自外周血的B细胞的有限稀释显示，约1/5-10可被活化以产生IgG、1/10 IgM、1/20-40伊加和1/2000-5000 IgE。在相关同种异体抗原刺激后，克隆也能够帮助缺乏同种异体抗原的B细胞，表明不需要直接的T-B细胞相互作用。该方法特别令人感兴趣，因为它适用于在不存在抗原的情况下由不受限制的T细胞辅助触发的B细胞亚群的克隆分析。
Human alloreactive helper T cell clones were isolated from a secondary mixed lymphocyte reaction by limiting dilution in the presence of irradiated stimulator cells and T cell growth factor (interleukin 2). When cultured with B cells and macrophages possessing the relevant alloantigens, the T cell clones proliferated and induced a strong B cell activation with production of high Ig levels. Limiting dilution of the B cells from the peripheral blood showed that about 1 in 5-10 can be activated to produce IgG, 1 in 10 IgM, 1 in 20-40 IgA and 1 in 2000-5000 IgE. Following stimulation by the relevant alloantigen, the clones were able to help also B cells that lacked the alloantigen, indicating that a direct T-B cell interaction is not required. This method is particularly interesting because it is suitable for the clonal analysis of a B cell subset that is triggered in the absence of antigen by an unrestricted T cell help.