Different ratios of eicosapentaenoicand docosahexaenoic omega-3 fatty acids in commercial fish oils differentially alter pro-inflammatory cytokines in peritoneal macrophages from C57BL/6 female mice
Different ratios of eicosapentaenoicand docosahexaenoic omega-3 fatty acids in commercial fish oils differentially alter pro-inflammatory cytokines in peritoneal macrophages from C57BL/6 female mice
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DOI：
10.1016/j.jnutbio.2006.02.005
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发表时间：
2007-01-01
影响因子：
5.6
通讯作者：
Fernandes, Gabriel
中科院分区：
文献类型：
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作者：
Bhattacharya, Arunabh;Sun, Dongxu;Fernandes, Gabriel
使用鱼油(FO)作为膳食补充剂来预防或减轻心血管疾病和自身免疫性疾病(如类风湿性关节炎)的严重程度正受到人们的广泛关注。最近的几份报告表明，经常吃鱼或使用小剂量的FO胶囊似乎对预防心血管疾病有好处。我们过去曾报道，与玉米油(CO)饮食相比，富含FO的饮食可以预防肾脏疾病，并延长自身免疫倾向小鼠的寿命。然而，商业FOS中二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)的最佳比例还没有很好地确定，以减少各种促炎细胞因子的产生。因此，我们从三个不同EPA/DHA含量的来源获得脱臭FO，以10%(体积/重量)的饲料(油A，11/10；油B，14/9；油C，23/14)喂养C57BL/6小鼠8周，并与(10%)共喂小鼠作为对照。用双抗体夹心酶联免疫吸附试验检测脂多糖处理后8h和2 4h的巨噬细胞中肿瘤坏死因子-α、白介素6和白介素1β的含量。结果表明，在油C中仅8h后，肿瘤坏死因子-α显著降低，24小时后，只有喂油C的小鼠血清中的肿瘤坏死因子-α、IL-6和IL-1β水平下降，而喂油A的小鼠与喂CO的小鼠相比，下降不明显。与饲喂CO的小鼠相比，饲喂C油的小鼠肾脏中的抗氧化酶过氧化氢酶和谷胱甘肽转移酶更高。这项研究表明，由于EPA/DHA含量的不同，不同来源的FO的抗炎活性可能不同。(C)2007 Elsevier Inc.保留所有权利。
The use of fish oil (FO) as a dietary supplement to prevent or reduce the severity of cardiovascular diseases and autoimmune disorders such as rheumatoid arthritis is receiving much attention. Several recent reports indicate that eating fish often or the use of small doses of FO capsules appears to have benefits against cardiovascular diseases. We have reported in the past that diets enriched with FO protect against renal diseases and prolong the life span of autoimmune-prone mice compared to corn oil (CO) diets. However, the optimum ratio of eicosapentaenoic acid (EPA) to docosahexaenoic acid (DHA) in commercially available FOs to reduce the production of various proinflammatory cytokines has not been well established. We, therefore, obtained deodorized FO from three sources containing different EPA/ DHA contents, fed them to C57BL/6 mice for 8 weeks in a 10% (vol/wt) diet (oil A, 11/10; oil B, 14/9; oil C, 23/14) and compared them with (10%) CO-fed mice as control. TNF-alpha, IL-6 and IL-1 beta were measured by enzyme-linked immunosorbent assay in thioglycollate-induced macrophages, 8 and 24 h after lipopolysaccharide treatment. The results showed a significant decrease in TNF-alpha after only 8 h in oil C. After 24 h, TNF-alpha, IL-6 and IL-1 beta levels decreased only in mice fed oil C, although nonsignificant decreases were seen in mice fed oil A compared to mice fed CO. The antioxidant enzymes, catalase and glutathione transferase, were higher in kidneys of mice fed oil C compared to mice fed CO. The study suggests that anti-inflammatory activity may vary among different sources of FO due to variations in EPA/DHA content. (c) 2007 Elsevier Inc. All rights reserved.