Optimization of NPK fertilization combined with phytoremediation of cadmium contaminated soil by orthogonal experiment
Optimization of NPK fertilization combined with phytoremediation of cadmium contaminated soil by orthogonal experiment
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正交试验优化氮磷钾施肥联合植物修复镉污染土壤
DOI：
10.1016/j.ecoenv.2019.109997
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发表时间：
2020
影响因子：
6.8
通讯作者：
Zhou Pei
中科院分区：
文献类型：
--
作者：
Wang Juncai;Chen Xunfeng;Chi Yaowei;Chu Shaohua;Hayat Kashif;Zhi Yuee;Hayat Sik;ar;Terziev Dimitar;Zhang Dan;Zhou Pei
研究了氮磷钾配比对镉污染土壤-植物龙葵（Solanum nigrum L.）系统以及植物修复效率进行了全面的研究。通过正交试验（L14）优化了Cd污染土壤可持续植物修复与农艺同步技术的氮磷钾（NPK）配比，以实现植物生产力和最大化植物修复潜力。结果表明，与对照相比，不同氮磷钾配比处理提高了土壤蔗糖酶、脲酶、过氧化氢酶和酸性磷酸酶的活性，但土壤pH值略有下降，降幅为3.64%~6.67%。植物生物量和地上部分（茎和叶）Cd浓度的变化。施用不同配比的NPK肥，可显著（P< 0.05）提高黑烟的产量，分别比对照提高14.19%~48.97%和38.50%~127.15%。与对照相比，施用NPK肥显著降低了Cd的根/冠比和转移系数，而增加了生物富集系数。不同氮磷钾配比的水稻叶片光合色素（叶绿素a、叶绿素B、总叶绿素和类胡萝卜素）和可溶性蛋白质含量均显著高于对照。抗坏血酸过氧化物酶（APX）、过氧化氢酶（CAT）、过氧化物酶（POD）、超氧化物歧化酶（SOD）和谷胱甘肽还原酶（GR）等抗氧化酶的活性升高，丙二醛（MDA）和脯氨酸含量降低。主成分分析（PCA）结果表明，N3 P2 K2处理综合得分最高，这是由于N3 P2 K2处理的土壤硫素组成最优。黑素系统此外，还发现肥料的最佳组合（N_3P_2K_2）导致植物对Cd的抗性和植物提取效率的提高。因此，建议所有的小农和科学界，应根据土壤性质、环境条件和Cd胁迫条件下植物的需求，精确地使用NPK肥料，以实现最大的生物量、Cd吸收效率以及Cd中度污染土壤的植物修复潜力。
In the current experiment, influence of NPK composition on the Cd contaminated soil-plant (Solanum nigrumL.) system as well as the phytoremediation efficiency were comprehensively studied. The composition of NPK was optimized for a sustainable phytoremediation and simultaneous agronomic technique in Cd-contaminated soil by orthogonal (L14) experiment, aimed to achieve plant productivity and maximum phytoremediation potential enhancement. Results showed that different treatments of NPK composition enhanced soil properties including saccharase, urease, catalase and acid phosphatase activities as compared to thecontrol treatment,however, soil pH was slightly decreased by 3.64%~6.67% with different composition of NPK treatments. Plant biomass and Cd concentration in the aboveground part (stem and leaves) ofS. nigrumwere significantly (P< 0.05) enhanced by 14.19%~48.97% and 38.50%~127.15% as compared to control plants with the addition of NPK fertilizers having different composition. Meanwhile, with the application of NPK fertilizer root/shoot Cd ratio and translocation factor (TF) was significantly decreased, however, bioconcentration factor (BCF) was increased as compared to control. Additionally, different composition of NPK fertilizers significantly increased photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids) and soluble protein in comparison to control. The activities of antioxidant enzymes inS. nigrumincluding ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and glutathione reductase (GR) were increased, while malonaldehyde (MDA) and proline contents were decreased. Principal component analysis (PCA) showed that N3P2K2treatment had the highest comprehensive score amongst other studied treatments of NPK composition, owing to its optimal composition for the investigated soil-S. nigrumsystem. Moreover, it was found that optimal composition (N3P2K2) of fertilizer resulted in increase of the plant resistance to Cd and the efficiency of phytoextraction. Therefore, it is suggested to all the small-holder famers and scientific community that precise composition of NPK fertilizer should be utilized according to soil properties, environmental conditions and plant requirements under Cd-stress condition in order to achieve maximum biomass, Cd uptake efficiency as well phytoremediation potential in moderately Cd contaminated soil.