Concentration of MHC class II molecules in lipid rafts facilitates antigen presentation
Concentration of MHC class II molecules in lipid rafts facilitates antigen presentation
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DOI：
10.1038/77842
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发表时间：
2000-08-01
影响因子：
30.5
通讯作者：
Roche, PA
中科院分区：
文献类型：
--
作者：
Anderson, HA;Hiltbold, EM;Roche, PA
真核细胞的质膜是不均匀的，并拥有独特的胆固醇和鞘脂丰富的筏微区，富含蛋白质已知是必不可少的细胞功能。脂筏微结构域对于T细胞受体（TCR）介导的T细胞活化是重要的。然而，脂筏对抗原呈递细胞（APC）的重要性及其在主要组织相容性（MHC）II类限制性抗原呈递中的作用尚未研究。发现MHC II类分子组成性地存在于B细胞的质膜脂筏中。这些微区的破坏显着抑制抗原呈递在有限浓度的抗原。筏破坏对抗原呈递的抑制作用可以通过用异常高剂量的抗原装载APC来克服，表明筏缔合将MHC II类分子浓缩成微结构域，其允许在低配体密度下有效的抗原呈递。
The plasma membranes of eukaryotic cells are not uniform and possess distinct cholesterol- and sphingolipid-rich raft microdomains that are enriched in proteins known to be essential for cellular function. Lipid raft microdomains are important for T cell receptor (TCR)-mediated activation of T cells. However, the importance of lipid rafts on antigen presenting cells (APCs) and their role in major histocompatibility (MHC) class II-restricted antigen presentation has not been examined. MHC class II molecules were found to be constitutively present in plasma membrane lipid rafts in B cells. Disruption of these microdomains dramatically inhibited antigen presentation at limiting concentrations of antigen. The inhibitory effect of raft disruption on antigen presentation could be overcome by loading the APCs with exceptionally high doses of antigen, showing that raft association concentrates MHC class II molecules into microdomains that allow efficient antigen presentation at low ligand densities.