Identification of new protein-protein interactions involving the products of the chromosome- and plasmid-encoded type IV secretion loci of the phytopathogen Xanthomonas axonopodis pv. citri
Identification of new protein-protein interactions involving the products of the chromosome- and plasmid-encoded type IV secretion loci of the phytopathogen Xanthomonas axonopodis pv. citri
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DOI：
10.1128/jb.187.7.2315-2325.2005
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发表时间：
2005-04-01
影响因子：
3.2
通讯作者：
Farah, CS
中科院分区：
文献类型：
--
作者：
Alegria, MC;Souza, DP;Farah, CS
植物病原细菌黄单胞菌致病变种Xanthomonaxonopodispv. Citri含有两个virB基因簇，一个在染色体上，一个在64 kb质粒上，每个基因簇编码先前未表征的IV型分泌系统（T4SS）。在这里，我们使用酵母双杂交试验，以确定在这两个系统中的蛋白质-蛋白质相互作用。我们的研究结果揭示了已知的T4 SS组分之间的相互作用，以及先前未表征的相互作用，涉及两个X中由开放阅读框架编码的假设蛋白质。轴足虫变种柑橘virB基因座我们的研究结果表明，这两个基因座可能编码以前未鉴定的VirB7蛋白，我们显示相互作用与VirB6或VirB9或与同一基因座编码的假设蛋白。此外，已经发现一组先前未表征的黄单胞菌属蛋白与VirD4相互作用，VirD4的基因与染色体virB基因座相邻。该家族的一个成员的基因被发现在染色体virB基因座内。所有这些未知的蛋白质在其C末端具有保守的120个氨基酸的结构域，并且可能代表黄单胞菌T4SS的辅因子或底物家族。
The recently sequenced genome of the bacterial plant pathogen Xanthomonas axonopodis pv. citri contains two virB gene clusters, one on the chromosome and one on a 64-kb plasmid, each of which codes for a previously uncharacterized type IV secretion system (T4SS). Here we used a yeast two-hybrid assay to identify protein-protein interactions in these two systems. Our results revealed interactions between known T4SS components as well as previously uncharacterized interactions involving hypothetical proteins coded by open reading frames in the two X. axonopodis pv. citri virB loci. Our results indicate that both loci may code for previously unidentified VirB7 proteins, which we show interact with either VirB6 or VirB9 or with a hypothetical protein coded by the same locus. Furthermore, a set of previously uncharacterized Xanthomonas proteins have been found to interact with VirD4, whose gene is adjacent to the chromosomal virB locus. The gene for one member of this family is found within the chromosomal virB locus. All these uncharacterized proteins possess a conserved 120-amino-acid domain in their C termini and may represent a family of cofactors or substrates of the Xanthomonas T4SS.