Synthesis, Characterization and Antioxidant Activity of Quaternized Carboxymethyl Chitosan Oligosaccharides
Synthesis, Characterization and Antioxidant Activity of Quaternized Carboxymethyl Chitosan Oligosaccharides
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季铵化羧甲基壳寡糖的合成、表征及抗氧化活性
DOI：
10.1080/10601325.2012.714679
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发表时间：
2012-01-01
影响因子：
2.5
通讯作者：
Sun, Runcang
中科院分区：
文献类型：
--
作者：
Li, Xiaoyun;Liu, B. O.;Sun, Runcang
为了研究壳聚糖的季铵基和羧甲基对壳聚糖抗氧化活性的影响，以壳聚糖为原料，以氯乙酸和2，3-环氧丙基三甲基氯化铵为改性剂，在微波辐射下制备了9种季铵化羧甲基壳寡糖（QCMCOs）。采用FT-IR、NMR、XRD等手段对QCMCO的结构进行了表征，并采用凝胶渗透色谱（GPC）测定了其分子量。通过热重分析（TGA）评价了其热稳定性，并考察了其抗氧化活性，包括清除超氧阴离子自由基和羟自由基的能力、还原能力和金属螯合能力。结果表明，季铵基和羧甲基的引入降低了壳寡糖的结晶度和热稳定性，其抗氧化活性与季铵基和羧甲基的取代度密切相关。本研究为开发新的抗氧化剂提供了重要指导。
In order to determine the effect of quaternary ammonium groups and carboxymethyl groups of chitosan on antioxidant activity, nine quaternized carboxymethyl chitosan oligosaccharides (QCMCOs) were prepared from chitosan with chloroacetic acid and 2,3-epoxypropyltrimethyl ammoniumchloride as the modifying agent under microwave irradiation. The structures of QCMCOs were characterized by FT-IR, NMR, XRD and their Mw were detected by gel permeation chromatography (GPC). The thermal stability was evaluated by thermal gravimetric analysis (TGA), and their antioxidant activities were investigated including scavenging activity of superoxide and hydroxyl radical, reducing power and metal chelating ability. The results revealed that the introduction of quaternary ammonium groups and carboxymethyl groups decreased the crystallinity and the thermal stability of chitosan oligosaccharide (COS), and their antioxidant activities were closely related to the degree of substitution of quaternary ammonium groups and the carboxymethyl groups. This study provides important guidelines for developing new antioxidant agents.