CADMIUM INTERFERES WITH STEROID-BIOSYNTHESIS IN RAT GRANULOSA AND LUTEAL CELLS-INVITRO
CADMIUM INTERFERES WITH STEROID-BIOSYNTHESIS IN RAT GRANULOSA AND LUTEAL CELLS-INVITRO
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DOI：
10.1007/bf01061225
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发表时间：
1992-12-01
期刊：
影响因子：
3.5
通讯作者：
LAZAR, P
中科院分区：
文献类型：
--
作者：
PAKSY, K;VARGA, B;LAZAR, P
最近，镉被描述为干扰大鼠的卵巢功能。 本文研究了镉对体外培养的卵巢细胞产生类固醇激素的直接影响。从发情前期和妊娠大鼠中获得颗粒细胞和黄体细胞，并在存在或不存在0.1-1000 ng ml-1促卵泡激素（FSH）或促黄体激素（LH）的情况下与0、5、10、20或40 μ g ml-1氯化镉孵育24或48 h。用放射免疫法测定颗粒细胞孕酮（P）和17 β-雌二醇（E2）的生成量及黄体细胞孕酮（P）的生成量。在FSH刺激的颗粒细胞培养物中，5和40 μ g ml-1氯化镉抑制P的积累，分别为65%和10%; E2的积累（在5 μ g ml-1氯化镉）下降到44%。P生产LH支持的黄体细胞下降到86和66%，分别为5和40 μ g ml-1氯化镉加入到培养基中。没有改变基础P积累发生在颗粒和黄体细胞培养物孵育后，20和40 μ g ml-1氯化镉，而基础E2生产的颗粒细胞显着减少。它的结论是，氯化镉抑制类固醇合成在体外产生直接影响颗粒和黄体细胞的功能。
Recently, cadmium has been described to disturb ovarian function in rats. In this paper the direct influence of cadmium on steroid production of ovarian cells in vitro has been studied. Granulosa and luteal cells were obtained from proestrous and pregnant rats, and incubated with 0, 5, 10, 20 or 40 mug ml-1 CdCl2 in the presence or absence of 0.1-1000 ng ml-1 follicle stimulating hormone (FSH) or luteinizing hormone (LH) for 24 or 48 h. Production of progesterone (P) and 17beta-estradiol (E2) by granulosa and that of P by luteal cells were measured by radioimmunoassay. In FSH-stimulated granulosa cell cultures, 5 and 40 mug ml-1 CdCl2 suppressed P accumulation to 65 and 10%, respectively; accumulation of E2 (at 5 mug ml-1 CdCl2) decreased to 44%. P production of LH-supported luteal cells dropped to 86 and 66%, respectively, when 5 and 40 mug ml-1 CdCl2 was added to the medium. No alteration in basal P accumulation occurred in granulosa and luteal cell cultures following incubations with 20 and 40 mug ml-1 CdCl2, whereas basal E2 production of granulosa cells was markedly diminished. It is concluded that CdCl2 suppressing steroid synthesis in vitro exerts a direct influence on granulosa and luteal cell function.