Immunological analysis and pathological examination of gnotobiotic mice monoassociated with Mycoplasma pneumoniae
Immunological analysis and pathological examination of gnotobiotic mice monoassociated with Mycoplasma pneumoniae
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DOI：
10.1099/jmm.0.007872-0
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发表时间：
2009-06-01
影响因子：
3
通讯作者：
Kamiya, Shigeru
中科院分区：
文献类型：
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作者：
Sekine, Hideaki;Taguchi, Haruhiko;Kamiya, Shigeru
虽然支原体肺炎通常被认为是一种预后良好的疾病，但病原性宿主免疫反应与其发生有关。在本研究中，使用鼻内感染肺炎支原体或接种肺炎支原体的无菌小鼠来检查免疫应答的致病意义。肺炎抗原（可溶性抗原和部分纯化的抗原）。在与M.肺炎，10（4）c.f.u.。M.在感染后2-28天分离每个肺的肺炎链球菌。感染后第2天和第7天，组织病理学检查显示血管周围出现淋巴细胞浸润的炎性改变。在鼻内接种可溶性抗原或部分纯化抗原（F6和F10抗原）的小鼠中，在接种后2天，组织病理学检测到中性粒细胞和淋巴细胞浸润。在接种F6抗原的小鼠中观察到严重的肺炎伴组织破坏。在所有治疗的小鼠中观察到内源性细胞因子表达中的γ干扰素（IFN-γ）显性应答。这些结果表明，在与M. pneumoniae感染的小鼠与M.肺炎抗原此外，研究表明IFN-γ在感染M. pneumoniae或接种其抗原。特别是，F6抗原被认为是诱导组织损伤的重要毒力因子，导致肺中淋巴细胞和中性粒细胞浸润，表明免疫应答与M.肺炎
Although mycoplasmal pneumonia has been generally considered to be a disease with good prognosis, a pathogenic host immune response has been associated with its occurrence. In the present study, the pathogenic significance of the immune response was examined using germfree mice either infected intranasally with Mycoplasma pneumoniae or inoculated with M. pneumoniae antigens (soluble antigen and partially purified antigen). In gnotobiotic mice monoassociated with M. pneumoniae, 10(4) c.f.u.. M. pneumoniae per lung were isolated 2-28 days after infection. Inflammatory changes with infiltration of lymphocytes were histopathologically detected in the perivascular area at 2 and 7 days after infection. In the mice intranasally inoculated with soluble antigen or partially purified antigens (F6 and F10 antigens), infiltration of neutrophils and lymphocytes was histopathologically detected at 2 days after inoculation. Severe pneumonia with tissue destruction was observed in the mice inoculated with F6 antigen. A gamma interferon (IFN-gamma) dominant response in endogenous cytokine expression was observed in all the treated mice. These results indicate that inflammatory changes in the lung tissue were prolonged in gnotobiotic mice monoassociated with M. pneumoniae compared with mice inoculated with M. pneumoniae antigen. In addition, it was shown that IFN-gamma plays an important role in the pathogenesis of pneumonia in mice either infected with M. pneumoniae or inoculated with its antigen. In particular, the F6 antigen has been considered to be an important virulence factor in terms of induction of tissue injury causing infiltration of lymphocytes and neutrophils in the lung, suggesting a close interaction between the immune response and the occurrence of M. pneumoniae pneumonia.