Direct energy transfer from conjugated polymer to DNA intercalated dye: Label-free fluorescent DNA detection
Direct energy transfer from conjugated polymer to DNA intercalated dye: Label-free fluorescent DNA detection
复制标题
从共轭聚合物到 DNA 嵌入染料的直接能量转移：无标记荧光 DNA 检测
DOI：
10.1016/j.colsurfb.2010.10.026
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发表时间：
2011-06-15
影响因子：
5.8
通讯作者：
Wang, Shu
中科院分区：
文献类型：
--
作者：
Wang, Bing;Yang, Qiong;Wang, Shu
DNA检测方法的发展在疾病诊断、基因靶向药物开发和分子生物学等领域具有重要意义。本文合成了一种新型的阳离子水溶性高分子高分子，其主链中含有芴基团和柔性乙烯基团，可用于无标记DNA检测。PFV的构象自由度使其与双链DNA （dsDNA）具有更强的相互作用，并优化了PFV与插入双链DNA的溴化乙啶（EB）之间过渡时刻的取向。在dsDNA中观察到从PFV（供体）到EB（受体）的高效FRET，并通过共轭聚合物良好的光捕获能力放大了EB的发射。通过测量插入DNA中的PFV和EB之间的FRET比率，可以探测PFV与DNA之间的相互作用。与其他基于FRET和共轭聚合物的DNA检测方法相比，我们的方法避免了染料标记DNA探针的合成，大大降低了成本和合成复杂性。PFV/dsDNA/EB系统以简单、快速和无标记的方式在DNA检测方面提供了有前途的应用。(C) 2010 Elsevier B.V.版权所有
The development of methods for DNA detection is of importance in disease diagnosis, gene-targeted drug discovery and molecular biology field. In this paper, we synthesize a new cationic water-soluble CP containing fluorene moiety and flexible ethylenic moiety in the backbone (PRO for label-free DNA detection. The conformational freedom of PFV provides stronger interactions with double-stranded DNA (dsDNA) and optimizes the orientation of transition moments between PFV and ethidium bromide (EB) intercalated in dsDNA. The efficient FRET from PFV (donor) to EB (acceptor) intercalated in dsDNA is observed and the emission of EB is amplified by the good light-harvesting ability of conjugated polymers. The interactions between PFV and DNA can also be probed by measuring the FRET ratio between PFV and EB intercalated in DNA. In comparison to other DNA detection assays based on FRET and conjugated polymers, synthesis of dye-labeled DNA probe is avoided in our method, which significantly reduces the cost and the synthetic complexity. The PFV/dsDNA/EB system provides promising applications on DNA detection with a simply, fast and label-free manner. (C) 2010 Elsevier B.V. All rights reserved.