Administration of recombinant IFN1 protects zebrafish (Danio rerio) from ISKNV infection
Administration of recombinant IFN1 protects zebrafish (Danio rerio) from ISKNV infection
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施用重组 IFN1 可保护斑马鱼 (Danio rerio) 免受 ISKNV 感染
DOI：
10.1016/j.fsi.2010.04.020
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发表时间：
2010-09-01
影响因子：
4.7
通讯作者：
He, Jianguo
中科院分区：
文献类型：
--
作者：
Li, Zhongsheng;Xu, Xiaopeng;He, Jianguo
本研究利用传染性脾肾坏死病毒（ISKNV）和斑马鱼模型系统，研究了重组斑马鱼干扰素1（zfrIFN 1）对急性病毒感染的抑制作用以及给药时间对其保护效果的影响。体内实验表明，在注射后24小时内，施用重组zfrINF 1上调了几种IFN刺激基因的表达，并且这些基因的表达水平在三天内下降到与对照鱼中的那些相似的正常水平。然而，两种病毒基因，主要衣壳蛋白和病毒蛋白48基因的转录被显著抑制至少三天，表明zfrIFN 1介导的先天性免疫效应的持续时间更长为了评估zfrIFN 1对ISKNV感染的保护效力，我们通过在感染之前或之后的不同时间点腹膜内（IP）注射zfrIFN 1来比较ISKNV感染的斑马鱼的相对存活百分比（RPS）。在病毒感染前24.6或0 h或病毒感染后6 h，IP注射1 μ g zfrIFN 1/g鱼体重显著提高鱼的存活率。然而.感染后24小时IP注射等剂量的zfrIFN 1没有对鱼提供显著的保护。我们的结果表明zfrIFN 1在抑制ISKNV急性感染和启动斑马鱼的先天免疫应答方面是有效的，但其有效性取决于给药的时间。这项研究表明，干扰素对DNA-病毒在鱼的第一次，并提供有关鱼类干扰素的疗效，将证明在可能的治疗应用有用的信息（C）2010爱思唯尔有限公司版权所有
In the present study, we used the infectious spleen and kidney necrosis virus (ISKNV) and zebrafish model system to investigate the inhibitory effect of recombinant zebrafish interferon 1 (zfrIFN1) on acute viral infection and the impact of time of zfrIFN1 administration on its protective efficacy. In vivo experiments showed that administration of recombinant zfrINF1 up-regulated expression of several IFN-stimulated genes within 24 h of injection, and expression levels of these genes dropped to normal levels similar to those in control fish within three clays However, the transcriptions of two viral genes, the major capsid protein and virus protein 48 genes, were significantly inhibited for at least three clays, indicating a longer duration of the zfrIFN1-mechated innate immune effect To evaluate the protective efficacy of zfrIFN1 against ISKNV infection, we compared the relative percentage survival (RPS) of ISKNV-infected zebrafish by intraperitoneally (IP) injecting the fish with zfrIFN1 at different time points before or after infection. IP injection with 1 mu g zfrIFN1/g fish body weight at 24.6 or Oh before virus infection or 6 h after virus infection significantly improved fish survival. However. IP injection with an equal dose of zfrIFN1 24 h post-infection did not provide significant protection to the fish Our results suggest that zfrIFN1 is potent in inhibiting ISKNV acute infection and initiating the innate immune response in zebrafish, but its efficiency depends on the time of administration This study shows the protective effects of interferon against a DNA-virus in fish for the first time and provides information about the efficacy of fish interferon that will prove useful in possible therapeutic applications (C) 2010 Elsevier Ltd All rights reserved