Tetracycline induces stabilization of mRNA in Bacillus subtilis.
Tetracycline induces stabilization of mRNA in Bacillus subtilis.
复制标题
四环素可诱导枯草芽孢杆菌中 mRNA 的稳定。
DOI：
10.1128/jb.184.4.889-894.2002
复制
发表时间：
2002
影响因子：
3.2
通讯作者：
Bechhofer,DavidH
中科院分区：
文献类型：
--
作者：
Wei,Yi;Bechhofer,DavidH
Thetet(L) gene ofBacillus subtilisconfers low-level tetracycline (Tc) resistance. Previous work examining the >20-fold-inducible expression oftet(L) by Tc demonstrated a 12-fold translational induction. Here we show that the other component oftet(L) induction is at the level of mRNA stabilization. Addition of a subinhibitory concentration of Tc results in a two- to threefold increase intet(L) mRNA stability. Using a plasmid-borne derivative oftet(L) with a large in-frame deletion of the coding sequence, the mechanism of Tc-induced stability was explored by measuring the decay oftet(L) mRNAs carrying specific mutations in the leader region. The results of these experiments, as well as experiments with aB. subtilisstrain that is resistant to Tc due to a mutation in the ribosomal S10 protein, suggest different mechanisms for the effects of Tc on translation and on mRNA stability. The key role of the 5" end in determining mRNA stability was confirmed in these experiments. Surprisingly, the stability of several otherB. subtilismRNAs was also induced by Tc, which indicates that addition of Tc may result in a general stabilization of mRNA.
