Suppression on plant-parasitic nematodes using a soil fumigation strategy based on ammonium bicarbonate and its effects on the nematode community.
Suppression on plant-parasitic nematodes using a soil fumigation strategy based on ammonium bicarbonate and its effects on the nematode community.
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DOI：
10.1038/srep17597
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发表时间：
2015-12-01
影响因子：
4.6
通讯作者：
Shen Q
中科院分区：
文献类型：
--
作者：
Su L;Ruan Y;Yang X;Wang K;Li R;Shen Q
在酸性沙质土壤中，植物寄生线虫严重阻碍了香蕉的生产。本研究探讨了以碳酸氢铵为基础的新型熏蒸剂在密闭条件下防治植物寄生线虫的可能性。此外，还在盆栽和田间试验中，采用基于形态和取食习性的分类方法和PCR-DGGE方法，研究了其对线虫群落的影响。结果表明，石灰(L)和碳酸氢铵(AB)按适宜添加量(L 0.857 g kg−1和AB 0.428 g kg−1)混合使用，比单独使用AB或使用等氮量的氢氧化铵(AH)和氰化钙(CC)具有更强的杀线虫能力。不同熏蒸剂对线虫群落有不同程度的影响，通过形态分类和食性分类，LAB显示出良好的植物寄生杀线虫能力，尤其是对线虫和根结线虫，对线虫具有较强的杀线虫能力。食腐动物和杂食性食肉动物对化学物质的直接影响比食菌动物更敏感。本研究在实验室的基础上探索了一种防治植物寄生线虫的新型熏蒸剂，为确保香蕉产业在全球范围内的发展提供了一种潜在的策略。
Banana production is severely hindered by plant-parasitic nematodes in acidic, sandy soil. This study investigated the possibility of applying a novel fumigation agent based on ammonium bicarbonate as a strategy for controlling plant-parasitic nematodes under sealed conditions. Moreover, its effects on the nematode community in pot and field experiments were also measured using morphology and feeding-habit based classification and the PCR-DGGE method. Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg−1 of L and 0.428 g kg−1 of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount. The nematode community was altered by the different fumigants, and LAB showed an excellent plant-parasitic nematicidal ability, especially for Meloidogyne and Rotylenchulus, as revealed by morphology and feeding-habit based classification, and for Meloidogyne, as revealed by the PCR-DGGE method. Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores. This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.