Identification and quantification of neuroactive N-acylethanolamines in cottonseed processing fractions
Identification and quantification of neuroactive N-acylethanolamines in cottonseed processing fractions
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棉籽加工组分中神经活性 N-酰基乙醇胺的鉴定和定量
DOI：
10.1007/s11746-003-0681-5
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
G. Gross
中科院分区：
文献类型：
--
作者：
K. Chapman;B. Venables;Emese Dian;G. Gross
N-酰基乙醇胺（NAE）包括已被鉴定为哺乳动物生理学中有效的内源性大麻素介质的脂质物质。在这里，我们已经分离，鉴定和定量的天然存在于棉籽精炼馏分中的NAE。在检查的组分中，NAE在成品餐中最普遍，总计约8.5 μg/g鲜重。经GC-MS鉴定，这些NAE分别为肉豆蔻酸（14∶0）、棕榈酸（16∶0）、硬脂酸（18∶0）、油酸（18∶1）和亚油酸（18∶2）的乙醇酰胺，其中NAE含量最高，为18 ∶2和16 ∶0。NAE没有检测到精炼，漂白，和精炼油，虽然它们存在于不太纯化的油馏分。在微摩尔浓度下，NAE 18 ∶2和NAE 16 ∶0对培养的小鼠神经元网络均表现出神经调节活性。由此可见，油籽加工馏分可能代表了生物活性NAE的一种新型天然来源。
N-Acylethanolamines (NAE) include lipid species that have been identified as potent endocannabinoid mediators in mammalian physiology. Here we have isolated, identified, and quantified the NAE naturally present in cottonseed refining fractions. Among the fractions examined, NAE were most prevalent in finished meal, totaling approximately 8.5 μg/g fresh weight. These NAE were identified by GC-MS as ethanolamides of myristic (14∶0), palmitic (16∶0), stearic (18∶0), oleic (18∶1), and linoleic (18∶2) acids, with NAE18∶2 and NAE16∶0 being the most abundant. NAE were not detected in refined, bleached, and deodorized oil, although they were present in less-purified oil fractions. At micromolar concentrations, both NAE18∶2 and NAE16∶0 exhibited neuromodulatory activities in murine neuronal networks cultured and evaluated on microelectrode arrays. It follows that oilseed processing fractions may represent a novel, natural source of bioactive NAE.
DOI：
10.1073/pnas.96.10.5780
发表时间：
1999-05-11
影响因子：
11.1
作者：
Zimmer, A;Zimmer, AM;Bonner, TI
通讯作者：
Bonner, TI