Birth following vitrification of a small number of human oocytes
Birth following vitrification of a small number of human oocytes
复制标题
DOI：
10.1093/humrep/14.12.3077
复制
发表时间：
1999-12-01
影响因子：
6.1
通讯作者：
Trounson, A
中科院分区：
文献类型：
--
作者：
Kuleshova, L;Gianaroli, L;Trounson, A
我们报告了一名47岁的受者在妊娠37周时，将四位体外受精（IVF）患者的成熟卵母细胞玻璃化后，生下一名健康的女婴。将17个卵母细胞用1-2基乙二醇（40%）和0.6 mol/l蔗糖（20.54%）在打开的吸管中玻璃化，玻璃化后11个卵母细胞存活，胞浆内单精子注射（ICSI）获得5个原核受精卵，3个胚胎移植给3例患者，其中2例为原卵母细胞供体，未建立妊娠。第三个胚胎在植入前诊断证实X、13、14、15、16、18、21和22号染色体整倍体后，捐赠给一名47岁的不孕妇女。鉴于传统的缓慢冷却方法冷冻人类卵母细胞的成功率相对较低，成功完成妊娠对进一步研究探索玻璃化冷冻保存人类卵母细胞的潜在益处是令人鼓舞的。
We report the birth of a healthy baby girl at 37 weeks gestation to a 47 year old recipient, after vitrification of mature oocytes from four in-vitro fertilization (IVF) patients. A total of 17 oocytes was vitrified in 1-2 yl of ethylene glycol (40%) and 0.6 mol/l sucrose (20.54%) in open pulled straws, Eleven oocytes survived after vitrification and five pronuclear zygotes were obtained after intracytoplasmic sperm injection (ICSI), Three embryos were transferred to three patients, two of whom were the original oocyte donors and pregnancy was not established. The third embryo was donated to a 47 year old infertile woman after preimplantation diagnosis had confirmed euploidy for chromosomes X, 13, 14, 15, 16, 18, 21 and 22. The successfully completed pregnancy is encouraging for further research to explore the potential benefits of vitrification for the cryopreservation of human oocytes, given the relatively low success of conventional freezing of human oocytes by slow cooling methods.