Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks
Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks
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DOI：
10.1086/520091
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发表时间：
2007-09-15
影响因子：
6.4
通讯作者：
Mitchell, Tim J.
中科院分区：
文献类型：
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作者：
Jefferies, Johanna M. C.;Johnston, Calum H. G.;Mitchell, Tim J.
肺炎链球菌溶血素是人类病原体肺炎链球菌的重要毒力因子。对 121 个肺炎链球菌临床分离株的 ply 基因进行序列分析，发现了许多等位基因。选择 22 个菌株进行进一步分析，发现了 14 个蛋白质等位基因。其中 5 个之前已报道过，其余 9 个是新颖的。使用细胞裂解物测定肺炎球菌溶血素蛋白的特异性溶血活性。 6株无溶血活性，其余16株均有不同程度的溶血。我们报告说，先前在血清型 1、序列类型 (ST) 306 分离株中报道的非溶血性等位基因也存在于属于 ST53 谱系的许多血清型 8 肺炎球菌分离株中。已知血清型 I 和 8 肺炎球菌与侵袭性疾病的爆发有关。因此，非溶血性肺炎球菌溶血素等位基因与爆发相关肺炎链球菌血清型的显性克隆相关。
Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty-two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype I and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak-associated serotypes of S. pneumoniae.