A Phytophthora capsici RXLR effector targets and inhibits the central immune kinases to suppress plant immunity
A Phytophthora capsici RXLR effector targets and inhibits the central immune kinases to suppress plant immunity
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DOI：
10.1111/nph.17573
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发表时间：
2021-07-22
期刊：
影响因子：
9.4
通讯作者：
Dou, Daolong
中科院分区：
文献类型：
--
作者：
Liang, Xiangxiu;Bao, Yazhou;Dou, Daolong
受体样胞浆激酶亚家族VII(RLCK-VII)蛋白是植物模式识别受体(PRR)复合体中的中枢免疫蛋白，它们协调一系列复杂的防御反应以抵御细菌和真菌病原体。然而，RLCK-VII在植物-卵菌相互作用中的作用尚未确定。辣椒疫霉是一种臭名昭著的卵菌病原体，可侵染许多农业上重要的蔬菜。在这里，我们报告了RXLR25的鉴定，RXLR25是辣椒疫霉菌毒力所必需的RXLR效应器。在植物中，RXLR25的表达显著提高了植物对疫霉病原菌的敏感性。RXLR25严重削弱了拟南芥中微生物模式诱导的免疫激活。我们进一步证明RXLR25与RLCK-VII蛋白相互作用。利用9个RLCK-VII高位突变体，我们观察到RLCK-VII-6和RLCK-VII-8是辣椒疫霉抗性所必需的成员。RLCK-VII-6成员是疫霉培养滤液(CF)诱导免疫反应所必需的。RXLR25直接靶向RLCK-VII蛋白，如BIK1、PBL8和PBL17，并抑制模式诱导的RLCK-VIIs的磷酸化，以抑制下游免疫反应。本研究确定了辣椒疫霉菌的一个关键毒力因子，并揭示了RLCK-VII蛋白在植物-卵菌相互作用中的重要性。
Receptor-like cytoplasmic kinase subfamily VII (RLCK-VII) proteins are the central immune kinases in plant pattern-recognition receptor (PRR) complexes, and they orchestrate a complex array of defense responses against bacterial and fungal pathogens. However, the role of RLCK-VII in plant-oomycete pathogen interactions has not been established. Phytophthora capsici is a notorious oomycete pathogen that infects many agriculturally important vegetables. Here, we report the identification of RXLR25, an RXLR effector that is required for the virulence of P. capsici. In planta expression of RXLR25 significantly enhanced plants' susceptibility to Phytophthora pathogens. Microbial pattern-induced immune activation in Arabidopsis was severely impaired by RXLR25. We further showed that RXLR25 interacts with RLCK-VII proteins. Using nine rlck-vii high-order mutants, we observed that RLCK-VII-6 and RLCK-VII-8 members are required for resistance to P. capsici. The RLCK-VII-6 members are specifically required for Phytophthora culture filtrate (CF)-induced immune responses. RXLR25 directly targets RLCK-VII proteins such as BIK1, PBL8, and PBL17 and inhibits pattern-induced phosphorylation of RLCK-VIIs to suppress downstream immune responses. This study identified a key virulence factor for P. capsici, and the results revealed the importance of RLCK-VII proteins in plant-oomycete interactions.