Sequence of two plasmids from Clostridium perfringens chicken necrotic enteritis isolates and comparison with C. perfringens conjugative plasmids.
Sequence of two plasmids from Clostridium perfringens chicken necrotic enteritis isolates and comparison with C. perfringens conjugative plasmids.
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DOI：
10.1371/journal.pone.0049753
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Prescott JF
中科院分区：
文献类型：
--
作者：
Parreira VR;Costa M;Eikmeyer F;Blom J;Prescott JF
26株不同MLST型产气荚膜梭菌（15株netB阳性，5株netB阴性）均含有1-4个大质粒，其中大多数netB阳性菌株含有3个大质粒，其数量和大小均大于netB阴性菌株。NetB和cpb 2在不同的质粒上发现，与以前的研究一致。包括netB在内的致病性基因座NELoc 1在这些质粒中基本上是保守的，而存在于含有cpb 2的质粒中的NeLoc 3则不那么保守。netB阳性和cpb 2阳性质粒可能是接合性的，并对质粒进行了完全测序。两种质粒都具有C.产气荚膜杆菌接合质粒。比较基因组分析的9个CpCP，包括两个质粒在这里描述的，显示了广泛的基因重排，包括致病位点和辅助基因插入周围，而不是在骨干区域。从这种分析中出现的模式是，各种毒力相关的CpCP的主要含毒素区域被组织为复杂的致病性基因座。这些不同但相关的CpCP如何在同一宿主中共存一直是一个未解的问题。这些质粒的复制分区的分析表明，该区域控制质粒的不相容性，CpCP可以分为至少四个不相容组。
Twenty-six isolates of Clostridium perfringens of different MLST types from chickens with necrotic enteritis (NE) (15 netB-positive) or from healthy chickens (6 netB-positive, 5 netB-negative) were found to contain 1–4 large plasmids, with most netB-positive isolates containing 3 large and variably sized plasmids which were more numerous and larger than plasmids in netB-negative isolates. NetB and cpb2 were found on different plasmids consistent with previous studies. The pathogenicity locus NELoc1, which includes netB, was largely conserved in these plasmids whereas NeLoc3, present in the cpb2 containing plasmids, was less well conserved. A netB-positive and a cpb2-positive plasmid were likely to be conjugative, and the plasmids were completely sequenced. Both plasmids possessed the intact tcp conjugative region characteristic of C. perfringens conjugative plasmids. Comparative genomic analysis of nine CpCPs, including the two plasmids described here, showed extensive gene rearrangements including pathogenicity locus and accessory gene insertions around rather than within the backbone region. The pattern that emerges from this analysis is that the major toxin-containing regions of the variety of virulence-associated CpCPs are organized as complex pathogenicity loci. How these different but related CpCPs can co-exist in the same host has been an unanswered question. Analysis of the replication-partition region of these plasmids suggests that this region controls plasmid incompatibility, and that CpCPs can be grouped into at least four incompatibility groups.
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影响因子：
6.4
作者：
Bannam TL;Yan XX;Harrison PF;Seemann T;Keyburn AL;Stubenrauch C;Weeramantri LH;Cheung JK;McClane BA;Boyce JD;Moore RJ;Rood JI
通讯作者：
Rood JI
影响因子：
6.7
作者：
Keyburn AL;Boyce JD;Vaz P;Bannam TL;Ford ME;Parker D;Di Rubbo A;Rood JI;Moore RJ
通讯作者：
Moore RJ
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M
影响因子：
64.8
作者：
Schumacher, Maria A.;Glover, Tiffany C.;Firth, Neville
通讯作者：
Firth, Neville
影响因子：
3.1
作者：
Li, Jihong;Miyamoto, Kazuaki;McClane, Bruce A.
通讯作者：
McClane, Bruce A.