Molecular analysis of sarcoidosis tissues for mycobacterium species DNA.
Molecular analysis of sarcoidosis tissues for mycobacterium species DNA.
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DOI：
10.3201/eid0811.020318
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发表时间：
2002-11
影响因子：
11.8
通讯作者：
Blaser MJ
中科院分区：
文献类型：
--
作者：
Drake WP;Pei Z;Pride DT;Collins RD;Cover TL;Blaser MJ
我们对25例结节病患者和25例对照组织标本进行了分枝杆菌16S rRNA、rpoB和IS6110序列的聚合酶链式反应分析，其中包括纵隔或颈部淋巴结和肺活检。从6例(24%)结节病标本中扩增出12例(48%)rpoB序列，扩增出分枝杆菌属16S rRNA序列。总共有15例(60%)结节病标本扩增出16SRRNA或rpoB序列，而在所有对照组织中均未检测到16SRRNA或rpoB序列(P=0.00002，卡方检验)。在三个样本中，序列类似于结核分枝杆菌以外的分枝杆菌物种。所有与结核分枝杆菌序列一致的样本IS6110均为阴性。我们提供的证据表明，在大多数结节病患者中都发现了一种分枝杆菌，特别是类似结核分枝杆菌的微生物。
We performed polymerase chain reaction analysis, for Mycobacterium species 16S rRNA, rpoB, and IS6110 sequences, on 25 tissue specimens from patients with sarcoidosis and on 25 control tissue specimens consisting of mediastinal or cervical lymph nodes and lung biopsies. Mycobacterium species 16S rRNA sequences were amplified from 12 (48%) rpoB sequences from 6 (24%) of the sarcoidosis specimens. In total, 16S rRNA or rpoB sequences were amplified from 15 sarcoidosis specimens (60%) but were not detected in any of the control tissues (p=0.00002, Chi square). In three specimens, the sequences resembled Mycobacterium species other than M. tuberculosis. All specimens with sequences consistent with M. tuberculosis were negative for IS6110. We provide evidence that one of a variety of Mycobacterium species, especially organisms resembling M. tuberculosis, is found in most patients with sarcoidosis.