ALBUMIN STANDARDS AND MEASUREMENT OF SERUM ALBUMIN WITH BROMCRESOL GREEN
ALBUMIN STANDARDS AND MEASUREMENT OF SERUM ALBUMIN WITH BROMCRESOL GREEN
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DOI：
10.1016/0009-8981(71)90365-2
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发表时间：
1971-01-01
影响因子：
5
通讯作者：
BIGGS, HG
中科院分区：
文献类型：
--
作者：
DOUMAS, BT;WATSON, WA;BIGGS, HG
本文介绍了溴甲酚绿绿色测定血清白蛋白的方法。向溴甲酚绿色的0.075 M琥珀酸盐缓冲液（pH 4.20）溶液中加入白蛋白，导致628 nm处吸光度增加。对于含高达6 g/dl白蛋白的样品，吸光度-浓度关系呈线性。胆红素、中度脂血症和水杨酸盐不干扰分析。使用非离子表面活性剂（Brij-35）可降低空白的吸光度，防止浑浊并提供线性。本法测定结果与电泳法和盐分级法测定结果一致。该方法操作简便，精密度好，试剂稳定，并介绍了一种可同时用于血清总蛋白和白蛋白测定的蛋白质标准品。
A rapid and reliable method for measuring serum albumin employing bromcresol green is described. The addition of albumin to a solution of bromcresol green in a 0.075 M succinate buffer pH 4.20 results in an increase in absorbance at 628 nm. The absorbance—concentration relationship is linear for samples containing up to 6 g/dl albumin. Bilirubin, moderate lipemia, and salicylate do not interfere with the analysis. The use of a nonionic surfactant (Brij-35) reduces the absorbance of the blank, prevents turbidity and provides linearity. The results by this method agree very well with those obtained by electrophoresis and salt fractionation. The method is simple, it has excellent precision and the reagents are stable.A protein standard is introduced which can be employed for both the total serum proteins and albumin determinations.