Localization of the diphtheria toxin receptor-binding domain to the carboxyl-terminal Mr approximately 6000 region of the toxin.
Localization of the diphtheria toxin receptor-binding domain to the carboxyl-terminal Mr approximately 6000 region of the toxin.
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DOI：
10.1016/s0021-9258(19)39118-5
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发表时间：
1990-05
期刊：
影响因子：
--
通讯作者：
J. Rolf;H. Gaudin;L. Eidels
中科院分区：
文献类型：
--
作者：
J. Rolf;H. Gaudin;L. Eidels
通过用羟胺特异性裂解白喉毒素的羧基端Mr=5982肽，并通过快速高效液相色谱纯化。通过结合十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析、与特定单克隆抗体的反应性以及氨基末端序列分析来确定肽的身份。 Mr = 5982 肽被证明可以保护高度毒素敏感的 Vero 细胞免受白喉毒素的致命作用。这种保护作用被证明是由于抑制了细胞毒性过程的第一步，即毒素与其受体的结合。这些结果强烈表明Mr=5982羧基末端区域（氨基酸残基482-535）是或包含白喉毒素的受体结合结构域。
The carboxyl-terminal Mr = 5982 peptide of diphtheria toxin was prepared by specific cleavage of the toxin with hydroxylamine and purified by fast performance liquid chromatography. The identity of the peptide was established by a combination of sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, reactivity with specific monoclonal antibodies, and amino-terminal sequence analysis. The Mr = 5982 peptide was shown to protect highly toxin-sensitive Vero cells from the lethal action of diphtheria toxin. This protection was shown to be due to inhibition of the initial step in the cytotoxic process, the binding of toxin to its receptor. These results strongly suggest that the Mr = 5982 carboxyl-terminal region (amino acid residues 482-535) is, or contains, the receptor-binding domain of diphtheria toxin.