Long-term imaging of dorsal root ganglia in awake behaving mice
Long-term imaging of dorsal root ganglia in awake behaving mice
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DOI：
10.1038/s41467-019-11158-0
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发表时间：
2019-07-12
影响因子：
16.6
通讯作者：
Yang, Guang
中科院分区：
文献类型：
--
作者：
Chen, Chao;Zhang, Jinhui;Yang, Guang
背根神经节（DRG）含有对躯体感觉至关重要的一级感觉神经元胞体。由于技术上的困难，清醒行为动物的DRG神经元活动仍然未知。在这里，我们开发了一种方法，在清醒的小鼠数周内以细胞和亚细胞分辨率成像DRG。该方法包括安装椎间融合支架以减少脊柱运动，以及植入椎体玻璃窗而不干扰动物的运动和感觉功能。在体内双光子钙成像显示，DRG神经元的活动是清醒的比麻醉动物。在足底福尔马林注射后，DRG神经元活动立即显著增加，并且这种活动激增与动物的阶段性疼痛行为相关。福尔马林注射后5周内重复DRG成像显示与持续疼痛相关的持续神经元过度活跃。本文所述的方法为在体研究DRG在感觉知觉和疾病中的功能提供了重要手段。
The dorsal root ganglia (DRG) contain the somas of first-order sensory neurons critical for somatosensation. Due to technical difficulties, DRG neuronal activity in awake behaving animals remains unknown. Here, we develop a method for imaging DRG at cellular and subcellular resolution over weeks in awake mice. The method involves the installation of an intervertebral fusion mount to reduce spinal movement, and the implantation of a vertebral glass window without interfering animals' motor and sensory functions. In vivo two-photon calcium imaging shows that DRG neuronal activity is higher in awake than anesthetized animals. Immediately after plantar formalin injection, DRG neuronal activity increases substantially and this activity upsurge correlates with animals' phasic pain behavior. Repeated imaging of DRG over 5 weeks after formalin injection reveals persistent neuronal hyperactivity associated with ongoing pain. The method described here provides an important means for in vivo studies of DRG functions in sensory perception and disorders.