Interleukin‐12 subunits p35 and p40 of Indian water buffalo (Bubalus bubalis) maintain high sequence homology with those of other ruminants
Interleukin‐12 subunits p35 and p40 of Indian water buffalo (Bubalus bubalis) maintain high sequence homology with those of other ruminants
复制标题
印度水牛 (Bubalus bubalis) 的 IL-12 亚基 p35 和 p40 与其他反刍动物保持高度序列同源性
DOI：
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发表时间：
2005
影响因子：
2.2
通讯作者：
T. Rasool
中科院分区：
文献类型：
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作者：
A. Premraj;E. Sreekumar;B. Nautiyal;T. Rasool
印度布法罗（Bubalus bubalis）是热带地区主要的奶畜之一，其免疫系统很少受到关注。从伴刀豆球蛋白A刺激的淋巴细胞中分离编码印度布法罗异二聚体白细胞介素（IL）-12的两个亚基的cDNA。通过逆转录聚合酶链反应（RT-PCR）扩增710-bp p35和1012-bp p40亚基，克隆、测序并与其他反刍动物序列进行比较。IL-12 p35亚基cDNA有9个核苷酸变异，与牛IL-12 p35具有98.1%的氨基酸同一性。IL-12 p40 cDNA具有13个核苷酸变异，与牛IL-12 p40具有97.5%的氨基酸同一性。与其他反刍动物IL-12分子相比，这两个亚基在预测的二级结构和关键氨基酸残基上显示出严格的保守性。大肠杆菌表达的布法罗IL-12 p40重组蛋白与牛抗IL-12 p40单克隆抗体发生交叉反应。我们的研究表明，这种关键细胞因子在反刍动物中具有很高的保守性。
The immune system of Indian water buffalo (Bubalus bubalis), one of the major dairy animals of the tropics, has received little attention. cDNAs encoding the two subunits of the heterodimeric interleukin (IL)‐12 of Indian water buffalo were isolated from concanavalin A‐stimulated lymphocytes. The 710‐bp p35 and 1012‐bp p40 subunits were amplified by reverse transcriptase polymerase chain reaction (RT‐PCR), cloned, sequenced and compared with other ruminant sequences. The IL‐12 p35 subunit cDNA had nine nucleotide variations and shared 98.1% amino acid identity with the cattle IL‐12 p35. The IL‐12 p40 cDNA had 13 nucleotide variations and had 97.5% amino acid identity with the cattle IL‐12 p40. Both the subunits showed strict conservation in the predicted secondary structure and critical amino acid residues compared with other ruminant IL‐12 molecules. Buffalo IL‐12 p40 recombinant protein expressed in Escherichia coli cross‐reacted with cattle anti‐IL‐12 p40 monoclonal antibody. Our study indicates a high level of conservation of this key cytokine among ruminants.