Communities of purple sulfur bacteria in a Baltic Sea coastal lagoon analyzed by puf LM gene libraries and the impact of temperature and NaCl concentration in experimental enrichment cultures
Communities of purple sulfur bacteria in a Baltic Sea coastal lagoon analyzed by puf LM gene libraries and the impact of temperature and NaCl concentration in experimental enrichment cultures
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DOI：
10.1111/j.1574-6941.2011.01175.x
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发表时间：
2011-12-01
影响因子：
4.2
通讯作者：
Imhoff, Johannes F.
中科院分区：
文献类型：
--
作者：
Tank, Marcus;Bluemel, Martina;Imhoff, Johannes F.
近岸浅水沃茨是光合紫硫细菌（PSB）经常大量繁殖的地方，其理化条件（包括温度和盐度）会发生巨大的昼夜变化和事件驱动的变化。为了分析光合细菌科普这些环境因素的能力，并在复杂的社区竞争，我们研究了波罗的海泻湖的光合细菌的环境群落的变化，在实验富集条件下，控制温度和NaCl浓度的变化。首次利用光合反应中心基因pufL和M，通过RFLP和克隆实验，对光合细菌群落内的变化进行了特异性分析。在生境中最丰富的光合细菌类型被发现沿着NaCl梯度从淡水条件下，7.5%的NaCl。伴随着数量较少的紫色非硫细菌和好氧不产氧光合细菌。微咸水泻湖光合细菌群落的主要组成部分属于海洋性、嗜盐性或耐盐性光合细菌属和种，包括M arichromatium、H alochromatium、T hiorhodococcus、A llochromatium、T hiocapsa、T hiorhodovibrio和T hiohalocapsa。一个戏剧性的转变发生在41和44摄氏度的高温时，M arichromatium gracile变得最突出，这是没有检测到在较低的温度。
Shallow coastal waters, where phototrophic purple sulfur bacteria (PSB) regularly form massive blooms, are subjected to massive diurnal and event-driven changes of physicochemical conditions including temperature and salinity. To analyze the ability of PSB to cope with these environmental factors and to compete in complex communities we have studied changes of the environmental community of PSB of a Baltic Sea lagoon under experimental enrichment conditions with controlled variation of temperature and NaCl concentration. For the first time, changes within a community of PSB were specifically analyzed using the photosynthetic reaction center genes pufL and M by RFLP and cloning experiments. The most abundant PSB phylotypes in the habitat were found along the NaCl gradient from freshwater conditions up to 7.5% NaCl. They were accompanied by smaller numbers of purple nonsulfur bacteria and aerobic anoxygenic phototrophic bacteria. Major components of the PSB community of the brackish lagoon were affiliated to PSB genera and species known as marine, halophilic or salt-tolerant, including species of M arichromatium, H alochromatium, T hiorhodococcus, A llochromatium, T hiocapsa, T hiorhodovibrio, and T hiohalocapsa. A dramatic shift occurred at elevated temperatures of 41 and 44 degrees C when M arichromatium gracile became most prominent which was not detected at lower temperatures.