Insights into the Mechanism of Proliferation on the Special Microbes Mediated by Phenolic Acids in the Radix pseudostellariae Rhizosphere under Continuous Monoculture Regimes.
Insights into the Mechanism of Proliferation on the Special Microbes Mediated by Phenolic Acids in the Radix pseudostellariae Rhizosphere under Continuous Monoculture Regimes.
复制标题
连续单一栽培下太子参根际酚酸介导的特殊微生物增殖机制的探讨
DOI：
10.3389/fpls.2017.00659
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发表时间：
2017
影响因子：
5.6
通讯作者：
Lin W
中科院分区：
文献类型：
--
作者：
Wu H;Xu J;Wang J;Qin X;Wu L;Li Z;Lin S;Lin W;Zhu Q;Khan MU;Lin W
酚酸类化合物作为一种有效的化感物质，被认为与太子参连作病害有关，可引起太子参根际土壤微生物区系的转移和结构的紊乱。利用转录组测序技术研究了甘蔗根分泌物中主要化感物质酚酸类对病原菌Kosakonia sacari和有益菌Bacillus pumilus的不同反应机制。太子参在单一栽培系统中的作用。本研究将微生物接种于含有土壤和药用植物的盆中。结果表明，添加有益B.结果表明，与未处理的2年生单作根际土壤相比，施用白僵菌显著降低了土壤脲酶、过氧化氢酶、蔗糖酶和纤维素酶的活性，提高了几丁质酶的活性。当K.加入蔗糖。转录组分析表明，香草醛增强了克雷伯氏菌的糖酵解/代谢、脂肪酸合成、戊糖磷酸、细菌趋化性、鞭毛组装和磷酸转移酶系统途径。糖。而K.在B中，从香草醛中提取蔗糖，对柠檬酸循环和新生霉素、苯丙氨酸、酪氨酸和色氨酸的生物合成具有负面影响。短小的。同时，原儿茶酸降低了B的生物膜形成。pumilus。这些结果揭示了酚酸如何差异介导根际土壤中微生物植物群的转移，导致病原细菌增殖（即，K.糖）和有益细菌的衰减（即，B。pumilus）在R.太子参
As potent allelochemicals, phenolic acids are believed to be associated with replanting disease and cause microflora shift and structural disorder in the rhizosphere soil of continuously monocultured Radix pseudostellariae. The transcriptome sequencing was used to reveal the mechanisms underlying the differential response of pathogenic bacterium Kosakonia sacchari and beneficial bacterium Bacillus pumilus on their interactions with phenolic acids, the main allelochemicals in root exudates of R. pseudostellariae in the monoculture system. The microbes were inoculated in the pots containing soil and the medicinal plant in this study. The results showed that the addition of beneficial B. pumilus to the 2-year planted soil significantly decreased the activity of soil urease, catalase, sucrase, and cellulase and increased the activity of chitinase compared with those in the 2nd-year monocropping rhizosphere soil without any treatment. However, opposite results were obtained when K. sacchari was added. Transcriptome analysis showed that vanillin enhanced glycolysis/gluconeogenesis, fatty acid biosynthesis, pentose phosphate, bacterial chemotaxis, flagellar assembly, and phosphotransferase system pathway in K. sacchari. However, protocatechuic acid, a metabolite produced by K. sacchari from vanillin, had negative effects on the citrate cycle and biosynthesis of novobiocin, phenylalanine, tyrosine, and tryptophan in B. pumilus. Concurrently, the protocatechuic acid decreased the biofilm formation of B. pumilus. These results unveiled the mechanisms how phenolic acids differentially mediate the shifts of microbial flora in rhizosphere soil, leading to the proliferation of pathogenic bacteria (i.e., K. sacchari) and the attenuation of beneficial bacteria (i.e., B. pumilus) under the monocropping system of R. pseudostellariae.