Isolation and characterization of an equine foamy virus
Isolation and characterization of an equine foamy virus
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DOI：
10.1128/jvi.74.9.4064-4073.2000
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发表时间：
2000-05-01
影响因子：
5.4
通讯作者：
Saïb, A
中科院分区：
文献类型：
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作者：
Tobaly-Tapiero, J;Bittoun, P;Saïb, A
泡沫病毒(FV)是从包括非人灵长类动物、牛和猫在内的不同动物物种中分离到的复杂逆转录病毒。在这里，我们报告了从马的血液样本中分离到的一种新的FV的分离和鉴定。与其他FV相似，马泡沫病毒(EFV)表现出高度特征性的超微结构，能在大量细胞系上诱导合胞体形成和随后的细胞裂解，分子克隆显示EFV的一般组织结构与其他已知FV相似，而与其牛FV同源基因的序列相似性仅为40%。有趣的是，EFV的发芽完全来自质膜，而不是来自内质网(ER)，正如先前对其他FV所示。EFV Env中缺乏ER检索双赖氨酸基序可能是导致这种意外分选途径的原因。
Foamy viruses (FVs) are complex retroviruses which have been isolated from different animal species including nonhuman primates, cattle, and cats. Here, we report the isolation and characterization of a new FV isolated from blood samples of horses. Similar to other FVs, the equine foamy virus (EFV) exhibits a highly characteristic ultrastructure and induces syncytium formation and subsequent cell lysis on a large number of cell lines, Molecular cloning of EFV reveals that the general organization is that of other known FVs, whereas sequence similarity with its bovine FV counterpart is only 40%. Interestingly, EFV buds exclusively from the plasma membrane and not from the endoplasmic reticulum (ER), as previously shown for other FVs. The absence of the ER retrieval dilysine motif in EFV Env is likely responsible for this unexpected sorting pathway.