Sialylated MUC1 mucin expression in normal pancreas, benign pancreatic lesions, and pancreatic ductal adenocarcinoma.
Sialylated MUC1 mucin expression in normal pancreas, benign pancreatic lesions, and pancreatic ductal adenocarcinoma.
复制标题
正常胰腺、良性胰腺病变和胰腺导管腺癌中唾液酸化 MUC1 粘蛋白的表达。
作者：
Y. Masaki;Masaaki Oka;Y. Ogura;T. Ueno;Kenji Nishihara;Akira Tangoku;M. Takahashi;Masakazu Yamamoto;T. Irimura
背景/目的
胰腺癌是所有胃肠道癌症中预后最差的。由于唾液酸化粘蛋白影响癌细胞的生物学行为，我们研究了胰腺导管腺癌患者中唾液酸化MUC 1粘蛋白的表达。
方法
检测55例胰腺导管腺癌、2例正常胰腺、3例慢性胰腺炎、1例胰腺导管增生、3例黏液性囊腺瘤和2例胰腺导管腺癌肝转移瘤中唾液酸化MUC 1的表达。用新的单克隆抗体（mAb）（MY.1E12）通过免疫组织化学评估表达。
结果
唾液酸化MUC 1粘蛋白在所有导管癌的癌细胞膜上表达。当这些细胞处于小管形成时，在细胞的顶端看到反应产物。粘液性囊腺瘤中也可检测到这种模式。然而，在单个癌细胞或小簇细胞中，以及在转移性肝肿瘤中，可在细胞膜上广泛观察到它。也就是说，侵入或转移的癌细胞在整个细胞膜上表达这种类型的粘蛋白。唾液酸化MUC 1粘蛋白的表达在正常胰腺、慢性胰腺炎或胰腺导管增生的标本中未观察到。在正常胰腺和这些病变中，唾液酸化MUC 1的唾液酸化表达仅限于腺泡和分泌型粘蛋白。
结论
唾液酸化的MUC 1粘蛋白在整个癌细胞膜上表达，可能是胰腺导管腺癌转移潜力的一个因素。
BACKGROUND/AIMS
Pancreatic cancer has the poorest prognosis of all gastrointestinal cancers. Because sialylated mucin influences the biologic behavior of carcinoma cells, we investigated sialylated MUC1 mucin expression in patients with pancreatic ductal adenocarcinoma.
METHODOLOGY
The expression of sialylated MUC1 mucin was examined in 55 pancreatic ductal adenocarcinomas, 2 normal pancreas specimens, 3 chronic pancreatitis specimens, 1 ductal hyperplasia of the pancreas, 3 mucinous cystadenomas, and 2 liver metastases from pancreatic ductal adenocarcinoma. Expression was assessed by immunohistochemistry with a new monoclonal antibody (mAb) (MY.1E12).
RESULTS
Sialylated MUC1 mucin was expressed in the cancer cell membrane in all the ductal carcinomas. The reaction product was seen at the apical aspect of cells when these were in tubule formation. This pattern was also detected in mucinous cystadenomas. However, it was seen diffusely in the cell membrane in single cancer cells or small clusters of cells without tubule formation and in metastatic liver tumors. Namely, invading or metastatic cancer cells expressed this type of mucin throughout the entire cell membrane. The expression of sialylated MUC1 mucin was not observed in specimens from normal pancreas, chronic pancreatitis, or ductal hyperplasia of the pancreas. In normal pancreas and these lesions, expression of sialylated Mession of sialylated MUC1 was limited to acini and secreted mucin.
CONCLUSIONS
Sialylated MUC1 mucin, which is expressed throughout the cancer cell membrane, may be a factor in the metastatic potential of pancreatic ductal adenocarcinoma.